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Blue (488 nm)

X. 525/50. 505. Violet (405 nm). 450/50. LSR II Configuration. *PE-Alexa 610 (630/30). *YFP if doing w/ GFP (540/20 525LP). *Hoechst Red (635LP 670LP). APC Alexa 633 Alexa 647 Live/Dead Far Red. AmCyan CFP BD Horizon V500 Live/Dead Yellow. PI 1 PE-Texas Red mCherry

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Blue (488 nm)

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  1. X 525/50 505 Violet (405 nm) 450/50 LSR II Configuration *PE-Alexa 610 (630/30) *YFP if doing w/ GFP (540/20 525LP) *Hoechst Red (635LP 670LP) APC Alexa 633 Alexa 647 Live/Dead Far Red AmCyan CFP BD Horizon V500 Live/Dead Yellow PI1 PE-Texas Red mCherry Live/Dead Red APC-Cy7 APC-H7 APC-Alexa 750 PerCP PerCP-Cy5.52 PE-Cy5.52 SSC Indo-1 (Blue) Live/Dead Aqua 710/50 670/30 780/60 488/10 X 530/30 695/40 610/20 685 755 685 505 595 Yellow-Green (561 nm) 50mW X Red (640 nm) 40mW Blue (488 nm) UV (355 nm) 20mW X 575 735 505 690 635 582/15 780/60 710/50 660/20 530/30 450/50 PE dTomato PE-Cy7 PE-Alexa 750 Alexa 700 APC-Cy5.5 PE-Cy5 PE-Alexa 647 7AAD1 Hoeschst Blue DAPI Alexa 350 Live/Dead Blue *DsRed (590/20) FITC GFP YFP Alexa 488 Venus Live/Dead Green Pacific Blue BD Horizon V450 Alexa 405 eFluor 450 Live/Dead Violet *Indo-1 Violet (405/20) *GFP if doing w/ YFP (510/20 495LP) Note: There may be other compatible fluorophores/dyes that are not listed here. Always check fluorochrome combinations prior to running an experiment!!! *Denotes filter change 17AAD and PI may be problematic depending on your fluorochrome panel. 2PerCP-Cy5.5 and PE-Cy5.5 cannot be combined.

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