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Applications of NGS

Sequencing in fish ecotoxicogenomics 生三甲 499431133 宋帛軒. Applications of NGS. Ecotoxicogenomics Next-generation sequencing Non-model fish. Platforms and Technology 454 genome sequencer-FLX TM Ion Torrent semiconductor sequencer SOLiD TM System Illumina/Solexa Genome analyzer PacBio RS.

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Applications of NGS

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  1. Sequencing in fish ecotoxicogenomics 生三甲 499431133宋帛軒 Applications of NGS

  2. Ecotoxicogenomics • Next-generation sequencing • Non-model fish

  3. Platforms and Technology • 454 genome sequencer-FLXTM • Ion Torrent semiconductor sequencer • SOLiDTM System • Illumina/Solexa Genome analyzer • PacBio RS

  4. Bioinformatics, extracting informative toxicological information from NGS • Gene ontology (GO) • Gene Set Enrichment Analysis (GSEA) • Pathway Analysis • Reverse Engineering

  5. Research in NON-MODEL FISH species using NGS: • In fish transcriptomic analyses • In fish evolution and physiology

  6. Conclusion • NGS is a great promise for toxicogenomics in non-model fish species. • As “$” decreases, the combination of several NGS platforms should facilitate whole genome sequencing project. • Expand our knowledge

  7. Reference: • Alvine C. Mehinto, Christopher J. Martyniuk, Daniel J. Spade, Nancy D. Denslow,Front Genet. 2012; 3: 62. Published online 2012 April 25. doi: 10.3389/fgene.2012.00062

  8. Thank you!

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