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RNAseq is a powerful tool for genome studies, allowing for detailed transcriptome analysis and the identification of new transcripts. Various RNA sequencing protocols, including total RNA sequencing, polyA RNA sequencing, and small RNA sequencing, cater to diverse research needs. Specialized techniques such as DSN treatment and ribosomal depletion enhance sequencing outcomes. This guide delves into RNAseq applications and methods, enabling researchers to effectively analyze gene expression and explore the intricacies of the genome.
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