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Synthetic Biology

Synthetic Biology. About Synthetic Biology. “The application of engineering design principles to do construction, characterization, and design of biological systems” C. Smolke Engineering design principles such as: Standardization Grammar implementation Design Implementation (CAD).

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Synthetic Biology

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  1. Synthetic Biology

  2. About Synthetic Biology • “The application of engineering design principles to do construction, characterization, and design of biological systems” C. Smolke • Engineering design principles such as: • Standardization • Grammar implementation • Design Implementation (CAD)

  3. iGEM & Registry • Registry of Standarized Biological Parts: repositorium of BioBrick compliant parts. • iGEM: the primary international competition on synthetic biology aimed at enriching and curating the Registry of Biological Parts. • Focus at undergraduate programs around the world, with support from recognized advisors

  4. Standards • The BioBrick Standard: • Prefix • Suffix • Scalability

  5. WiFi Coli

  6. Goal & Approach • Goal: communication while deprecating chemical / spatial barrier • Approach: use a non-chemical messenger, i.e. photons • Encoding: have wavelength encoding • Parts: use existing available machinery

  7. Platform • Use BioBrick standard to: • assemble BioParts into modules, • organize modules into devices, • express devices within systems. BBa_I15008 BBa_I15008 BBa_I15009 BBa_I15010 BBa_I15009 BBa_I15010

  8. Platform • Use BioBrick standard to: • assemble BioParts into modules, • organize modules into devices, • express devices within systems. BBa_I15008 BBa_I15008 BBa_I15009 BBa_I15010 BBa_I15009 BBa_I15010 Regulator BBa_I15008 BBa_I15009 BBa_I15010 Terminator

  9. Platform • Use BioBrick standard to: • assemble BioParts into modules, • organize modules into devices, • express devices within systems. BBa_I15008 BBa_I15008 BBa_I15009 BBa_I15010 BBa_I15009 BBa_I15010 Regulator BBa_I15008 BBa_I15009 BBa_I15010 Terminator

  10. Organization • Emission • Transmission • Reception ReceptionModule Emission Module

  11. Constructions

  12. Expanded Behavior • Recycling pathway for substrate • Inducible expression of enzyme, ergo light

  13. Characterization Reception Module PoPs Photons Transfer Function Emission Module Photons PoPs Transfer Function

  14. Applications

  15. Trinary Cycle Red Emit. Blue Recep. Red Recep. Green Emit. Blue Emit. Green Recep.

  16. Control Cycle Rec. Module Emis. Module X

  17. Logic Gates & Processor Input Output

  18. FireFlyLuciferase Bacterial Luciferase

  19. LRERecyclingEnzyme

  20. MagicQuiralityConversion

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