1 / 10

Raster Image Correlation Spectroscopy

Raster Image Correlation Spectroscopy. Seminario di relazione per la partecipazione a: 3rd European Workshop on Advanced Fluorescence Imaging and Dynamics (8-12 Ottobre 2012) Di Paolo Pozzi. FCS. Raster Imaging. I. t. How to do RICS. Spatial autocorrelation:. Fitting Function.

duncan-aguilar
Télécharger la présentation

Raster Image Correlation Spectroscopy

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Raster Image Correlation Spectroscopy Seminario di relazione per la partecipazione a: 3rd European Workshop on Advanced Fluorescence Imaging and Dynamics (8-12 Ottobre 2012) Di Paolo Pozzi

  2. FCS

  3. Raster Imaging I t

  4. How to do RICS Spatial autocorrelation:

  5. Fitting Function Diffusive Component: Scanning Component:

  6. What could go wrong Scan too slow Undersampling Phototube shot noise is time correlated!!!

  7. What could go wrong Immobile population: RICS profile Uniform concentration and uniform diffusion coefficient are needed for rics!!!

  8. Immobile removal Subtracting averange value Obtaining negative value An arbitrary constant can be added, but the information is partially lost

  9. Number of images and image area 100 nM EGFP

More Related