RFLPRestriction Fragment Length Polymorphism Marie Černá, Markéta Čimburová, Marianna Romžová
RFLP –Restriction Fragment Length Polymorphism restriction analysis of DNA by its digestion with restriction endonucleases (RE) in specific restriction sites
RFLP –Restriction Fragment Length Polymorphism in the case the sequence difference (polymorphism) creates or disturbs a specific site for RE, after restriction, fragments with different sizes are formed
Principle • starting DNA (genomic DNA, PCR product) • digestion with a restriction enzyme into fragments with different sizes • fragments electrophoresis separation
Restriction endonucleases (RE) • known about 2100 bacterial RE • RE recognize variously short nucleotides sequences (4,6,8), in which then they digest covalent phosphodiester bonds
??? • Do RE often digest, which recognize shorter or longer sequences? • What is a role of RE in natural systems?