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RFLP Restriction Fragment Length Polymorphism

RFLP Restriction Fragment Length Polymorphism

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RFLP Restriction Fragment Length Polymorphism

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  1. RFLPRestriction Fragment Length Polymorphism Marie Černá, Markéta Čimburová, Marianna Romžová

  2. RFLP –Restriction Fragment Length Polymorphism restriction analysis of DNA by its digestion with restriction endonucleases (RE) in specific restriction sites

  3. RFLP –Restriction Fragment Length Polymorphism in the case the sequence difference (polymorphism) creates or disturbs a specific site for RE, after restriction, fragments with different sizes are formed

  4. Principle • starting DNA (genomic DNA, PCR product) • digestion with a restriction enzyme into fragments with different sizes • fragments electrophoresis separation

  5. Restriction endonucleases (RE) • known about 2100 bacterial RE • RE recognize variously short nucleotides sequences (4,6,8), in which then they digest covalent phosphodiester bonds

  6. ??? • Do RE often digest, which recognize shorter or longer sequences? • What is a role of RE in natural systems?