LABORATORY CONDUCT AND SAFETY REGULATIONS (Indoor & Field Safety)

1. LABORATORY CONDUCT AND SAFETY REGULATIONS(Indoor & Field Safety) Biology Lake City High School Mrs. Whittington

2. Standard Standard B-1 The student will demonstrate an understanding of how scientific inquiry and technological design, including mathematical analysis, can be used appropriately to pose questions, seek answers, and develop solutions. • B-1.9 • Use appropriate safety procedures when conducting investigations. Safety regulations are established for each laboratory by Federal, State, and School mandates.

3. Objectives Successful work in the laboratory and field involves not only mastery of concepts and techniques, but also knowing: • what the safety requirements are, • what equipment is available, and • when and how to use each piece of equipment • how to keep yourself and others safe and • how to respond to emergencies. Safety regulations are established for each laboratory by Federal, State, and School mandates.

4. Universal precautions and basic lab safety rules will be strictly enforced

5. NON-COMPLIANCE • 1st violation: leave the activity area until compliant • student may receive a zero for work missed • 2nd violation: leave the activity area for the day • student may receive a zero for all work missed that day

6. NON-COMPLIANCE • 3rd violation: leave the activity area permanently • student may receive a failing lab grade • *Severe violations will warrant immediate removal from the lab • student may receive a failing lab grade

7. Lab Safety

8. STUDENT BEHAVIOR • No horseplay (pushing, shoving, etc...) in the lab • Always be aware of potential hazards & act cautiously in the lab

9. Read, read, read • BEFORE beginning a lab READ ALL safety precautions and lab instructions..

10. Eating & Drinking • Food and drink of any kind are strictly prohibited in the lab.

11. Report all medical conditions (pregnancy, cardiac or respiratory disorders, allergies....) • Only authorized people in the lab. • All human secretions will be treated as biohazards

12. Stow books, backpacks, purses, etc... under the desk

13. APPAREL • Lab aprons (tied in back) • Long hair must be tied back • Only closed toed shoes

14. Proper Clothing Tie back loose hair! Avoid floppy clothing! Store belongings out of the way!

15. Never take any lab materials out of the lab. • Lab materials are costly and may be dangerous as well. • Taking them may result in school discipline or even legal prosecution.

16. Eye and Face Safety

17. No contact lenses during wet labs (when using chemicals or biohazards) • Safety glasses during all wet labs • Disposable gloves when working with hazards or chemicals

18. Eye Protection O Z87 Type Goggles

19. NO Food, Drink, Tobacco, Cosmetic use

20. Biology I Bell Ringer • You notice in the lab instructions that you will be using test tubes. What are two rules that you know you will need to remember? (Hint: There may be more than 2, but you only need to write about two.)

21. Bell Ringer Free Write Friday • Write at least 3 sentences on whatever you like. • Examples: Tell me how your first week at school has been. Have any plans for the weekend? Be prepared to turn in your bell ringers.

22. Today • Reminder Safety Contracts due today. • You may turn it in late for a point reduction. • Anyone miss yesterday’s pre-test? • Everyone must take the pre-test • Safety Quiz – Postponed due to printer issues. Will be on Tuesday! • Put standards page in your folder. Initial the safety indicator (B 1.9) • 1st Period – Acceptable Use Policy!

23. Assignment • You will be assigned a rule from the safety contract. • Your mission is to create a sign that will remind people to follow that rule. • Put your first and last name and the class period in the upper right hand corner. • Must communicate the rule • Must be creative and neat • Must finish in class

24. Standard Standard B-1 The student will demonstrate an understanding of how scientific inquiry and technological design, including mathematical analysis, can be used appropriately to pose questions, seek answers, and develop solutions. • B-1.9 • Use appropriate safety procedures when conducting investigations. Safety regulations are established for each laboratory by Federal, State, and School mandates.

25. Objectives Successful work in the laboratory and field involves not only mastery of concepts and techniques, but also knowing: • what the safety requirements are, • what equipment is available, and • when and how to use each piece of equipment • how to keep yourself and others safe and • how to respond to emergencies. Safety regulations are established for each laboratory by Federal, State, and School mandates.

26. Glassware Safety

27. Special Caution-Handling Glassware Protect hands. Lubricate glass surfaces. NEVER force the glassware!

28. Special Caution-Handling Glassware Check for any cracks, bubbles, or rough areas before using.

29. Broken Glass Disposal UNCONTAMINATED broken glass

30. Fire Safety

31. Watch for FLAMES from burners • No unauthorized or unsupervised experiments • Immediately report ALL • accidents • injuries • spills

32. FIRE SAFETY • Fire Extinguishers: • Aim nozzle at the base of the fire • Pull pin and squeeze handles together.

33. Fire Blanket: • Pull the blanket out of the bottom using the black cord • carefully cover the person to smother the fire

34. Fire Alarm: • Pull the handle on the fire alarm to activate. • Exit the building

35. Emergency exit route: • Exit the lab and building. • Meet the teacher at the designated site • Do NOT leave until you are released

36. Sharp Instruments

37. SharpsHandle with Care!

38. Sharps Disposal • Examples: • Needles • Razor blades • Scalpels • Scalpel blades

39. INSTRUCTIONS FOR WASTE CLEANUP

40. CHEMICAL WASTE DISPOSAL Dilution is NOT the solution to pollution!!!

41. LAB CLEAN-UP • Hands must be washed after removing gloves and before leaving the lab • Glassware must be washed with soap & water & rinsed with distilled water • Clean & disinfect your work area BEFORE leaving lab. • Everything must be returned to its proper place

42. WASTE DISPOSAL • All waste has a specific disposal container • Always use the properly labeled container • If unsure ask the teacher.

43. Don’t pour ANYTHING down the sink unless you are instructed to do so.

44. Biohazard Waste Labeling • LABEL ALL CONTAINTERS before putting anything in them with the following: • Date • Organism (or substance) • Medium (or chemical) • Your initials & class period

45. Know what waste products will be generated. • Chemical Waste Labeling: • Obtain a clean, dry, empty container. • Place a new CHEMICAL WASTE label on front.

46. DO NOT mix liquid & solid waste. • Liquid waste SHOULD NOT be mixed unless it is of the same type. • Fill container just below neck of the bottle.

47. Biohazard Disposal • A BIOHAZARD IS ANTYING THAT HAS COME INTO CONTACT WITH • BACTERIA • FUNGI • VIRUSES • BODILY FLUIDS

48. Plastic Biohazard Bag • Examples: • Petri dishes • Specimen containers • Pipette tips • contaminated gloves

49. Biohazard Disposal Boxes • Examples: Contaminated • microscope slides • coverslips • broken glass • pipettes • test tubes

50. Cardboard Biohazard Box • Examples: • Wooden probes • Toothpicks