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This study investigates the impact of leucine and calcitriol on C2C12 muscle cells, analyzing fluorescence intensity as a measure of protein expression. The experimental groups include control, leucine treatment, calcitriol treatment, and combinations of these with nife. By quantifying protein levels (μg), we aim to understand the interactive effects of these compounds on muscle cell metabolism and signaling pathways. The findings may contribute to nutritional strategies for muscle health and recovery.
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A Control Leucine Calcitriol B C2C12 alone * *** ** Fluorescence Intensity/ protein (μg) * Nife Control Leucine Leu+nife Calcitriol Calcitriol+nife