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Space Seed Experiment “Life Cycle of Higher Plants under Microgravity Conditions”

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Space Seed Experiment “Life Cycle of Higher Plants under Microgravity Conditions”

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  1. Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA). JEM SPACE SEED 31407 Space Seed Experiment “Life Cycle of Higher Plants under Microgravity Conditions” Instructor: Takashi YamasakiToru Mori (yamasaki.takashi@jaxa.jp)(mori.tooru@jaxa.jp)

  2. Objectives After this lesson the trainee will be able to: • Space Seed experiment overview • Space Seed experiment operations flow • Key points during Space Seed experiment operations • Space Seed experiment operations • Explain the science objective of Space Seed experiment. • List onboard outcomes from performing Space Seed experiment. • Explain function of Plant Exp Unit. • Explain function of Exp Laptop Terminal (ELT). • Explain Space Seed operations flow regarding Plant Exp Unit. • Explain function of KSC Fixation Tube (KFT). • Explain what crew must check after attaching Plant Exp Unit on CBEF Turntable. • Explain what crew must do in case fragments of plant tissue fly out of Glove Bag. • Explain what crew must check before opening KFT Ziplock Bag. • Explain what crew must take care while inserting plants in KFT. • Explain what crew must take care while taking Plant Exp Sample Chamber out of Plant Exp Unit. • Identify where crew must cut while harvesting plants. • Explain what crew must not do while inserting plants in KFTs to prevent cross-contamination. • Perform Space Seed experiment operations according to ODFs without instructor’s assistance.

  3. Lesson Outline Contents: - Space Seed Experiment Overview - Space Seed Experiment Operations Flow - Key Points during Space Seed Experiment - Appendix Page: 4 8 10 11

  4. Space Seed Experiment Overview Space Seed experiment science objective To study Life Cycle of Higher Plants under Microgravity Conditions. Onboard Outcomes from Space Seed experiment • Plants grown in Space (Arabidopsis thaliana) - Plant Exp Sample Chamber (4) - Plant Exp Sample Chamber Bottom (4) - KFT (12) (Kennedy Space Center Fixation Tube) (RNAlater (4), Formaldehyde (2), Fromalin Acetic Acid Ethanol (2), Glutaraldehyde Paraformaldehyde (2), Glutaraldehyde Paraformaldehyde for Long Term (2)) • Environment Gas - Plant Exp Gas Sampling Syringe (2) • Data - Temperature/Humidity Data - Video Images - Radiation Data - Microgravity Environment Data

  5. Bio Dosimeter Temperature Logger Space Seed Experiment Overview (cont’d) Plant Exp Unit - Plant Exp Unit is a kind of CBEF Canister to grow plants.- Plant Exp Unit has following components. - Plant Exp Sample Chamber - LEDs for growing plants - LEDs for lighting and a video camera - Water bag, Water Sensor and Water Supply Pump - Temperature/Humidity Sensor, Ventilation Pump, Heater and Circulation Fan - Plant Exp Sample Chamber also has following components. - Bio Dosimeter - Temperature Logger Ventilation Pump Video Camera Water Supply Pump Water Sensor Circulation Fan LED for lighting Heater Water Bag LED for growing plants Temperature/Humidity Sensor Plant Exp Chamber

  6. Space Seed Experiment Overview (cont’d) Exp Laptop Terminal - Exp Laptop Terminal is a laptop computer for controlling Plant Exp Unit in CBEF. Plant Exp Units in CBEF Micro-G Incubator Unit Plant Exp Units in CBEF 1G Incubator Unit Installed in CBEF Incubator Units Temperature/Humidity of Sample Chamber, Pump/Fan status, etc. Communication Check Experiment start/stop, etc. Plant Exp Unit Exp Laptop Terminal (ELT) (Think Pad A31p) ELT Data Cable Parameter files Exp data files Plant Exp Unit activation/deactivation Commands Ground Plant Exp Unit on/off status, Temp of circuit board Cell Biology Experiment Facility (CBEF)

  7. Intentionally Blank

  8. LEGEND: :Crew operations :Ground operations Tasks just before or during Long Term Experiment Dehumidifier Exchange Water Supply/Gas Sampling Space Seed Experiment Operations Flow Plant Exp Unit MMA Exp Laptop Terminal CBEF Plant Exp Unit Installation (8) MLT Activation Dehumidifier Installation ELT Setup/Activation Measurement Start CBEF Activation/Environment Control Plant Exp Unit Before Short Term experiment Plant Exp Unit Activation PEU Program Installation Experiment Start by ELT -Cultivation (Short Term: approx. 30 days) (Long Term: approx. 60 days) -Temperature/Humidity Data -Video Image -Radiation Data Dehumidifier -Microgravity Environment Data ELT Check (periodically) Repeat for Long Term Experiment Experiment Stop by ELT Exp Laptop Terminal Plant Exp Unit Deactivation After Long Term Experiment Plant Exp Unit Removal (4) Measurement Stop Gas Sampling Syringe After Long Term Experiment MLT Deactivation CBEF Deactivation Harvest (Short/Long Term) ELT Deactivation/Removal Dehumidifier Removal MELFI Insertion

  9. Space Seed Experiment Operations Flow (Cont’d) Space Seed Harvest Kit Harvest Take out Sample Chambers from Plant Exp Units Take out Sample Chamber Put Sample Chambers, KFTs, and Harvest Kit in Glove Bag Open Sample Chamber Harvest plants Open Sample Chamber Put plants in KFTs Glove Bag Fix plants by KFTs KSC Fixation Tube Take out Sample Chambers, KFTs, and Harvest Kit from Glove Bag Put plants in KFT Fix plants Harvest plants

  10. Key Points during Space Seed Experiment CAUTION: Payload Safety Review Panel has not been completed yet. NOTE: Underlined key points have been covered by CBEF Advanced Training.

  11. Appendix Appendix - Harvest for Short Term Experiment - Harvest for Long Term Experiment - Harvest in case of KFT leakage - Acronyms

  12. Harvest for Short Term Experiment Harvest for Short Term experiment (approx. 30 days) MELFI -95 deg C Trash CBEF Micro-G Incubator Unit Sample Chamber Bottom (4) Sample Chamber Top (4) KFT Formaldehyde (2) KFT RNAlater (4) A side B side S/N 001 Half of B side Relocation 5 days later CBEF 1G Incubator Unit A side Half of B side KFT RNAlater (4) S/N 003 A side KFT Formalin Acetic Acid Ethanol (2) B side S/N 005 Half of B side Remain in CBEF Incubator Unit for Long Term Experiment A side Half of B side MELFI 2 deg C KFT Glutaraldehyde Paraformaldehyde (2) S/N 007

  13. CBEF Micro-G Incubator Unit CBEF 1G Incubator Unit Harvest for Long Term Experiment Harvest for Long Term experiment (approx. 60 days) MELFI -95 deg C Sample Chamber Bottom (4) S/N 004 S/N 008 Half of B side KFT Formaldehyde (2) KFT RNAlater (4) S/N 004 Sample Chamber Bottom (2) S/N 002 KFT Formalin Acetic Acid Ethanol (2) KFT Glutaraldehyde Paraformaldehyde for Long Term (2) Sample Chamber Top (2) S/N 008 KFT Glutaraldehyde Paraformaldehyde (2) Stowed in MELFI from Short Term Experiment S/N 006 MELFI 2 deg C Sample Chamber (4)

  14. Harvest in case of KFT leakage Harvest in case of KFT RNALater leakage • - There are 5 KFT RNALater (4 used, 1 spare). • - If 1 KFT RNALater has leakage, use spare KFT RNALater. • If 2 KFT RNALater have leakage, plant samples from 2 Plant Exp Sample Chamber A side (S/N 005, 007) will be fixed in 1 KFT RNA Later. MELFI -95 deg C CBEF Micro-G Incubator Unit KFT RNAlater (3) A side S/N 001 Relocation 5 days later CBEF 1G Incubator Unit A side KFT RNAlater (3) S/N 003 A side S/N 005 Remain in CBEF Incubator Unit for Long Term Experiment A side MELFI 2 deg C S/N 007

  15. Harvest in case of KFT leakage (Cont’d) Harvest in case of KFT Formaldehyde leakage - There are 3 KFT Formaldehyde (2 used, 1 spare). - If 1 KFT Formaldehyde has leakage, use spare KFT Formaldehyde. MELFI -95 deg C CBEF Micro-G Incubator Unit KFT Formaldehyde (2) B side S/N 001 CBEF 1G Incubator Unit B side S/N 005 Remain in CBEF Incubator Unit for Long Term Experiment MELFI 2 deg C

  16. Harvest in case of KFT leakage (Cont’d) Harvest in case of KFT Formalin Acetic Acid Ethanol leakage - There are 2 KFT Formalin Acetic Acid Ethanol (2 used). - If 1 KFT Formalin Acetic Acid Ethanol has leakage, plant sample from B side of Plant Exp Sample Chamber S/N 007 will be fixed by KFT Glutaraldehyde Paraformaldehyde. MELFI -95 deg C CBEF Micro-G Incubator Unit Half of B side CBEF 1G Incubator Unit Half of B side S/N 003 KFT Formalin Acetic Acid Ethanol (1) Remain in CBEF Incubator Unit for Long Term Experiment MELFI 2 deg C B side KFT Glutaraldehyde Paraformaldehyde (2) S/N 007

  17. Harvest in case of KFT leakage (Cont’d) Harvest in case of KFT Glutaraldehyde Paraformaldehyde leakage - There are 2 KFT Glutaraldehyde Paraformaldehyde (2 used). - If 1 KFT Glutaraldehyde Paraformaldehyde has leakage, plant sample from B side of Plant Exp Sample Chamber S/N 007 will be fixed by KFT Formalin Acetic Acid Ethanol. MELFI -95 deg C CBEF Micro-G Incubator Unit Half of B side CBEF 1G Incubator Unit Half of B side S/N 003 KFT Formalin Acetic Acid Ethanol (2) B side Remain in CBEF Incubator Unit for Long Term Experiment MELFI 2 deg C KFT Glutaraldehyde Paraformaldehyde (1) S/N 007

  18. CBEF Micro-G Incubator Unit CBEF 1G Incubator Unit Harvest in case of KFT leakage (Cont’d) Harvest in case of KFT Glutaraldehyde Paraformaldehyde for long term leakage - There are 2 KFT Glutaraldehyde Paraformaldehyde for long term (2 used). - If 1 KFT Glutaraldehyde Paraformaldehyde for long term has leakage, plant sample from half of B side of Plant Exp Sample Chamber S/N 008 will be fixed by KFT Formaldehyde spare for short term experiment. MELFI -95 deg C Stowed in MELFI from Short Term Experiment KFT Formaldehyde (2) S/N 004 Half of B side KFT Formaldehyde (1) (Spare for short term) Sample Chamber Bottom (1) S/N 008 S/N 004 Half of B side S/N 002 Sample Chamber Bottom (1) KFT Glutaraldehyde Paraformaldehyde for Long Term (1) Sample Chamber Top (2) S/N 008 MELFI 2 deg C Sample Chamber (4) S/N 006

  19. CBEF ELT KSC KFT LED MMA MLT MELFI ODF Cell Biology Experiment Facility Experiment Laptop Terminal Kennedy Space Center KSC Fixation Tube Light Emitting Diode Microgravity Measurement Apparatus MMA Laptop Terminal Minus Eighty degree Laboratory Freezer for ISS Operations Data File Acronyms

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