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PhD students : BENZHA Fatima DAIFE Zineb EL YAMLOULI Karim

« Unravelling and Exploiting Mediterranean Sea Microbial Diversity and Ecology for Xenobiotics and Pollutants clean up ». PhD students : BENZHA Fatima DAIFE Zineb EL YAMLOULI Karim . Mr. BLAGHEN Mohammed Mr. FAHDE Abdelilah. Case Study «The lagoon of Mar Chika » . Nador.

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PhD students : BENZHA Fatima DAIFE Zineb EL YAMLOULI Karim

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  1. « Unravelling and ExploitingMediterraneanSeaMicrobialDiversity and Ecology for Xenobiotics and Pollutants clean up » PhDstudents: BENZHA Fatima DAIFE Zineb EL YAMLOULI Karim Mr. BLAGHENMohammed Mr. FAHDE Abdelilah

  2. Case Study«The lagoon of Mar Chika» Nador Laggon of Marc Chika Nador The lagoon of Mar Chika

  3. The Lagoon of Mar Chika (35°08'52''N, 2°50'53''O) has an area of ​​112 Km2, it is linked to the Mediterranean Sea by an artificial pass which is far from the shore by 7,5km. Located on the northwest coast of Morocco Mediterranean in front of the Alboran Sea between the towns of BeniEnzar and KariatArkman. Isolated from the Mediterranean by a strip of fine sand and dunes (25Km), while it had a communication channel to the sea by the key of Boucana. MediterraneanSea

  4. Materials & Methods Sampling strategy Physicochemicalanalysis Bacteriologicalstudy

  5. 1- Samplingstrategy Samples (water and sediment) were carried from 15 sites (10 P; 5 NP)

  6. PhysicochemicalAnalysis • Parameters measured in situ: pH, conductivity, temperature, dissolvedoxygen, salinity.

  7. PhysicochemicalAnalysis • Parameters measured in the laboratory: Chemical oxygendemand (COD), Alkaliniy (P.alkalinity, M.alkalinity), Calcium hardness, Total Hardness, Dosage of chloride. J. Rodier, B. Legube, N. Merlet (Juin2009) .L'analyse de l'eau: Eaux naturelles, eaux résiduaires, eau de mer

  8. Chemicaloxygendemand (COD): is a measure of the capacity of water to consume oxygen during the decomposition of organic matter and the oxidation of inorganic chemicals such as ammonia and nitrite. COD measurements are commonly made on samples of waste waters or of natural waters contaminated by domestic or industrial wastes. . Parametersmeasuredin the laboratory: • Alkalinity : isdefined as the measure of the water capacity to neutralizeacids. There are two types of alkalinity : • Phenolphtalein Alkalinity • Methyl Orange Alkalinity Aminot A.; Chausspied M (1983): « Manuel des analyses chimiques en milieu marin » Organichttp://science.jrank.org/pages/1388/Chemical-Oxygen-Demand.html#ixzz1Qh0kSt3N

  9. Parametersmeasuredin the laboratory: Calciumhardness of water : is the concentration of Ca2+ in the water and generally represents a significant portion of total hardness. Total Hardness: is an indicator of mineralization of the water. It is the sum of the concentrations of metal cations with the exception of alkali metals and hydrogen ion, and it is mostly proportional to the concentration of calcium and magnesium which sometimes are added to the concentrations of iron, aluminum, manganese and strontium. Aminot A.; Chausspied M (1983). « Manuel des analyses chimiques en milieu marin » Organichttp://science.jrank.org/pages/1388/Chemical-Oxygen-Demand.html#ixzz1Qh0kSt3N

  10. Bacteriologicalstudy • Microbiological analysis is based on research of bacteria considered as indicators of water contamination. • The quality of sampling is an essential step for good analysis. • The sample must be taken in a sterilized bottle in a specific way to prevent accidental contamination or variation in the quality of the bacteria present at sampling.

  11. Results«Parametersmeasured in situ »

  12. Results«Parametersmeasured in laboratory »

  13. Thepollutedsite n° 4

  14. The bacterial part revealed, at first, the existence of different bacterial strains that can be distinguished referring to nude eye observation. White Orange

  15. Following the study, we will identify the isolated bacteria and perform the same analysis in marine sediment.

  16. Thank you for your attention

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