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100. a. TNF. IL6. 80. 60. Positive cells. (%). 40. 20. 0. CD89. CD16b. CD1a. CD3. CD19. CD14. B. A. LPS. LPS. t tol = 8 h. t rec = 16 h. t st. Analysis (tol). LPS. t st. Analysis (c). D. C. 300. 40. IL12p35/p70. IL10. *. 30. 200. Positive cells.

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## Gomez-Piña et al., Supplemental Figure S1

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**100**a TNF IL6 80 60 Positive cells (%) 40 20 0 CD89 CD16b CD1a CD3 CD19 CD14 B A LPS LPS ttol = 8 h trec = 16 h tst Analysis (tol) LPS tst Analysis (c) D C 300 40 IL12p35/p70 IL10 * 30 200 Positive cells [Protein] (ng/ml) (%) 20 100 * * 10 * 0 0 c c + LPS tol + LPS c c + LPS tol + LPS Gomez-Piña et al., Supplemental Figure S1**A**8 1 -1 3 * * 6 6 -actin] x 10 18 4 24 * * * b 48 h * 2 [IL6/ 0 c c+ LPS tol + LPS tol + LPS (GM6001) B 4 1 -1 3 * 3 6 -actin] x 10 18 2 24 b * 48 h * 1 [IL23p19/ * * * 0 c c+ LPS tol + LPS tol + LPS (GM6001) Gomez-Piña et al., Supplemental Figure S2**A**4 3 6 h 3 -actin] x 10 * 2 * b / a 1 [TNF 0 c s'TREM-1 (50 ng/ml) c s'TREM-1 (10 ng/ml) s'TREM-1 (50 ng/ml) +LPS +LPS +LPS B 2.5 -2 3 2.0 6 h * -actin] x 10 * 1.5 b * 1.0 [IL12p40/ 0.5 0.0 c s'TREM-1 (50 ng/ml) c s'TREM-1 (10 ng/ml) s'TREM-1 (50 ng/ml) C 2.5 * 3 -2 2.0 6 h * 1.5 -actin] x 10 1.0 b 0.5 [IL10/ 0.0 c s'TREM-1 (50 ng/ml) c s'TREM-1 (10 ng/ml) s'TREM-1 (50 ng/ml) Gomez-Piña et al., Supplemental Figure S3

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