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Single-Molecule Imaging Reveals Active Transport of Receptors in Live Cells

This study employs single-molecule imaging techniques in digitonin-permeabilized cells to unveil the receptor populations that are engaged in active transport within live cells. The analysis focuses on kymographs documenting the simultaneous live cell imaging of TagRFP-T-IFT88 (RFP-IFT88, IFT trains) and the single molecule SSTR3 receptor. This approach provides valuable insights into the dynamics and mechanisms of receptor transport, enhancing our understanding of cellular signaling and function. This exploration delves into the complexities of membrane receptor behavior in live cell environments.

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Single-Molecule Imaging Reveals Active Transport of Receptors in Live Cells

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  1. Single molecule imaging in digitonin-permeabilized cells reveals the receptor population undergoing active transport in live cells.(A) Kymographs of simultaneous live cell imaging of TagRFP.T-IFT88 (RFP-IFT88, IFT trains) and single molecule SSTR3... Ye F et al. eLife Sciences 2013;2:e00654

  2. Single molecule imaging in digitonin-permeabilized cells reveals the receptor population undergoing active transport in live cells.(A) Kymographs of simultaneous live cell imaging of TagRFP.T-IFT88 (RFP-IFT88, IFT trains) and single molecule SSTR3... Ye F et al. eLife Sciences 2013;2:e00654

  3. Single molecule imaging in digitonin-permeabilized cells reveals the receptor population undergoing active transport in live cells.(A) Kymographs of simultaneous live cell imaging of TagRFP.T-IFT88 (RFP-IFT88, IFT trains) and single molecule SSTR3... Ye F et al. eLife Sciences 2013;2:e00654

  4. Single molecule imaging in digitonin-permeabilized cells reveals the receptor population undergoing active transport in live cells.(A) Kymographs of simultaneous live cell imaging of TagRFP.T-IFT88 (RFP-IFT88, IFT trains) and single molecule SSTR3... Ye F et al. eLife Sciences 2013;2:e00654

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