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Thr67 Occupied

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Thr67 Occupied

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  1. Supplemental Figures 1-101: Compilation of MS2 spectra for all detected peptides relating to the four O-glycosites in LDLR class A repeat linker regions, in three cell lines, with annotations by Proteome Discoverer 1.4. Figures include ”CID-MS2” and, where available, ETD-MS2 spectra for peptides in linker regions with occupied O-glycosites, and HCD-MS2 spectra for peptides with unoccupied O-glycosites. In some cases peptides with unoccupied O-glycosites are represented by ETD-MS2 or ”CID-MS2” spectra. Cell lines represented are HEK293 SC, HEK293 SC T11-KO, and CHO WT. O-glycosites include Thr67, Thr108, Thr147, and Thr235. Annotations of fragments derived by H2O loss from primary cleavage products have been omitted for clarity.

  2. HEK293 SC Thr67 Occupied Supplemental Figure #1 2

  3. HEK293 SC Thr67 Occupied Supplemental Figure #2

  4. HEK293 SC Thr67 Occupied Supplemental Figure #3

  5. HEK293 SC Thr67 Occupied Supplemental Figure #4

  6. HEK293 SC Thr67 Occupied Supplemental Figure #5

  7. HEK293 SC Thr67 Occupied Supplemental Figure #6

  8. HEK293 SC Thr67 Occupied Supplemental Figure #7

  9. HEK293 SC Thr67 Occupied Supplemental Figure #8

  10. HEK293 SC Thr67 Occupied Supplemental Figure #9

  11. HEK293 SC Thr67 Occupied Supplemental Figure #10

  12. HEK293 SC Thr67 Unoccupied Supplemental Figure #11

  13. HEK293 SC Thr67 Unoccupied Supplemental Figure #12

  14. HEK293 SC Thr67 Unoccupied Supplemental Figure #13

  15. HEK293 SC Thr67 Unoccupied Supplemental Figure #14

  16. HEK293 SC Thr67 Unoccupied Supplemental Figure #15

  17. HEK293 SC Thr67 Unoccupied Supplemental Figure #16

  18. HEK293 SC Thr67 Unoccupied Supplemental Figure #17

  19. HEK293 SC Thr108 Occupied (inferred but not unambiguously determined, due to lack of ETD-MS2) Supplemental Figure #18

  20. HEK293 SC Thr108 Occupied (inferred but not unambiguously determined, due to lack of ETD-MS2) Supplemental Figure #19

  21. HEK293 SC Thr108 Occupied (inferred but not unambiguously determined, due to lack of ETD-MS2) Supplemental Figure #20

  22. HEK293 SC Thr108 Occupied (inferred but not unambiguously determined, due to lack of ETD-MS2) Supplemental Figure #21

  23. HEK293 SC Thr108 Occupied (inferred but not unambiguously determined, due to lack of ETD-MS2) Supplemental Figure #22

  24. HEK293 SC Thr108 Occupied (inferred but not unambiguously determined, due to lack of ETD-MS2) Supplemental Figure #23

  25. HEK293 SC Thr108 Unoccupied Supplemental Figure #24

  26. HEK293 SC Thr108 Unoccupied Supplemental Figure #25

  27. HEK293 SC Thr108 Unoccupied Supplemental Figure #26

  28. HEK293 SC Thr108 Unoccupied Supplemental Figure #27

  29. HEK293 SC Thr108 Unoccupied Supplemental Figure #28

  30. HEK293 SC Thr147 Occupied Supplemental Figure #29

  31. HEK293 SC Thr147 Occupied Supplemental Figure #30

  32. HEK293 SC Thr147 Occupied Supplemental Figure #31

  33. HEK293 SC Thr147 Unoccupied Supplemental Figure #32

  34. HEK293 SC Thr147 Unoccupied Supplemental Figure #33

  35. HEK293 SC Thr147 Unoccupied Supplemental Figure #34

  36. HEK293 SC Thr235 Occupied Supplemental Figure #35

  37. HEK293 SC Thr235 Occupied Supplemental Figure #36

  38. HEK293 SC Thr235 Occupied Supplemental Figure #37

  39. HEK293 SC Thr235 Occupied Supplemental Figure #38

  40. HEK293 SC Thr235 Occupied Supplemental Figure #39

  41. HEK293 SC Thr235 Occupied Supplemental Figure #40

  42. HEK293 SC Thr235 Occupied Supplemental Figure #41

  43. HEK293 SC Thr235 Occupied Supplemental Figure #42

  44. HEK293 SC Thr235 Occupied Supplemental Figure #43

  45. HEK293 SC Thr235 Occupied Supplemental Figure #44

  46. HEK293 SC Thr235 Occupied Supplemental Figure #45

  47. HEK293 SC Thr235 Occupied Supplemental Figure #46

  48. HEK293 SC Thr235 Occupied Supplemental Figure #47

  49. HEK293 SC Thr235 Unoccupied Supplemental Figure #48

  50. HEK293 SC Thr235 Unoccupied Supplemental Figure #49