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Image for bead in water at z=60

These are some images that were obtained with the new algorithm Changes in the gray scale doesn’t show any change in the image

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Image for bead in water at z=60

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  1. These are some images that were obtained with the new algorithm Changes in the gray scale doesn’t show any change in the image The resolution used was the same for both, object and psf, however we can not see anything in the images when the thickness object is large. The First object used was the binary target The NA=075 In this case the increment for xy plane (spatial) was and for z axis was xy XZ

  2. Image for bead in water at z=60 These are the xz images for glass bead in oil XY image Considering the zeropadding facto then the image should be z axis X axis

  3. Image for bead in oil at z=60 These same experiments were done using different NA and different Index of refraction en the objects.

  4. Why it is happening • when we are doing three-dimensional convolution with are doing • superposition of each plane of the specimen with each value of PSF • in the spatial domain and z axis. Then contributions of all planes are added • to each plane of interest, so object in a specific plane are affected by these • contributions. • In Preza’s paper the model was tested using objects with small thickness, • It could be a reason why the images in the binary target doesn’t look to bad. • In another reference show how we can remove the effect of different planes • Out focus in the plane where the object is in focus. • It could be a features to add to de model.

  5. Binary Target Images

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