MAKING NEW BODY PARTS BIOT 412, 2011 student
Making a New Bladder • Neurogenic bladder disorder • Cystoplasty; a surgical treatment • What we did??? • Human bladder was engineered!
How??? • Perform bladder biopsy; for urothelial and muscle cells. • In vitro culturing and expansion of cells. • Seeding of cells on a biodegradable bladder-shaped scaffold. • New organ was ready after 8 weeks.
Contd… • 5. Anastomosis • 6. Apply fibrin glue. • 7. Omental wrapping. Anastomosis
Results • After 46 months: • Improved function & capacity of ureter. • Renal function was normal through out the follow up. • No metabolic complication occured.
Positives: • No immuno-suppression required! • Negatives: • No immediate Reconstruction of Vascular supply. • Instant Oxygen and nutrients supply is by diffusion. • Inflammation before re-angiogenesis.
Making a New Trachea • End stage bronchio-malacia in a 30 year old woman, for example. • What we did??? • Ex- vivo bioengineering of trachea.
How??? • Retrieving of deceased donor trachea. • Decellularization. • Obtaining epithelial cells from bronchial mucosa. • Isolation of stem cells from patient’s bone marrow. • Differentiation of SCs into chondrocytes. • 4 days maturation. • Human trachea was used as natural scaffold. • Seeding with autologous epithelial cells and chondrocytes. • Implantation of construct to replace the diseased one.
Results • After 18 months: • Imaging showed normal architecture. • Patient was doing well. • Advantage! • Use of natural/ human scaffold.