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  1. Supplementary Figure S4 (A.) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 1,900 Kb 1,640 Kb 1,120 / 1,100 Kb 945 Kb 915 Kb 815 Kb 680 Kb 610 Kb 555 Kb 450 Kb 375 Kb 295 Kb 225 Kb 1 2 3 4 5 6 7 (B.) 1,900 / 1,640 Kb 1,120 / 1,100 Kb 945 Kb 915 Kb 815 Kb 680 Kb 555 Kb 450 Kb 225 Kb

  2. Supplementary Figure S5 (A.) Fragment size L 3-1 3-2 3-3 3-4 3-5 3-6 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 1 2 3 4 5 6 7 8 bp 875 775 670 570 470 370 1000 bp 800 bp 1,900 / 1,640 Kb 600 bp 500 bp 1,120 / 1,100 Kb 400 bp 300 bp 945 Kb 280 150 915 Kb 200 bp 815 Kb 100 bp 680 Kb L 3-1 3-2 3-3 3-4 3-5 3-6 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 555 Kb bp 920 820 720 620 520 420 450 Kb 1000 bp 800 bp 225 Kb 600 bp 500 bp 400 bp 300 bp 320 220 120 200 bp 100 bp L 3-1 3-2 3-3 3-4 3-5 3-6 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 bp 1000 500 1000 bp 800 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp

  3. Supplementary Figure S5 (B.) PMM1294 – (471 bp) PMM0039 - (609 bp) L V 1-13 2-2 2-19 3-2 3-3 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 V 1-13 2-2 2-19 3-2 3-3 3-7 3-8 L 1000 bp 500 bp 300 bp 100 bp PMM0039 - (609 bp) PMM0214 - (1656 bp) L 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 V 1-13 2-2 2-19 3-2 3-3 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 L 1000 bp 500 bp 300 bp 100 bp PMM0214 - (1656 bp) PMM1600 - (1368 bp) L V 1-13 2-2 2-19 3-2 3-3 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 L V 1-13 3-15 1000 bp 500 bp 2000 bp PMM0844 - (1173 bp) 1650 bp L V 1-13 2-2 2-19 3-2 3-3 3-7 3-8 3-9 3-10 3-11 3-12 3-13 3-14 3-15 3-16 1000 bp 1000 bp 500 bp

  4. Supplementary Figure S6 -HIS -HIS-LYS -HIS-URA -HIS-LEU -HIS-TRP V 1-13 2-2 2-19

  5. Supplementary Figure S7 S. cerevisiae: MED4

  6. Supplementary Chart 1