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The Effect of Alcohol on Lumbriculus variegates

The Effect of Alcohol on Lumbriculus variegates. By: Martha Vallejo and Jasmyn Smith.

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The Effect of Alcohol on Lumbriculus variegates

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  1. The Effect of Alcohol on Lumbriculus variegates By: Martha Vallejo and Jasmyn Smith Introduction: This experiment that we have conducted was created to measure the affect of a said toxin on Lumbriculus variegates on their pulse rate. Lumbriculus variegates, alsoreferred to as California blackworm. The worms have transparent skin, which makes it easier to analyze the pulse rate. In this experiment, the pulse rates were analyzed over a duration of time, and then compared. Alcohol is described as a depressant drug; it elevates blood levels and significantly affects the central nervous system of an organism. The molecular formula for alcohol, or Ethanol is CH3CH2OH. In this experiment, we used 40% ethanol alcohol in our solution, making our solution a 1mM solution. Hypothesis: If the worms are put in a alcohol solution then they will have a slower pulse rate than the worms in the distilled water. Procedure: Step 1: Label cups (distilled, alcohol, distilled recovery, alcohol recovery) and fill with 4mL of said solution. Step 2: Place 10 worms in the alcohol for 15 minutes and then place each worm on the well slide, using microscope 10x, count the pulse rate for 30 seconds in the midsection of the worm. (Repeat for each worm). Step 3: Record data (double for a minute count) and place worm in recovery cup. Step 4: Repeat steps to measure pulse rate with worms in distilled water. Remove each to recovery cup. Results: The average pulse rate for the worms in distilled water is 21.2. We found that the average pulse rate for the worms in the alcohol solution is 7.4. Conclusion: After analyzing the data, we found that our hypothesis does in fact support our data. The data shows us that the worms reacted to the depressant just as we thought. A common pulse rate for worms in distilled water was 20. A common pulse rate for worms in the alcohol solution was between 4 and 8. We also had 2 worms within the alcohol solution die. While calculating the pulse rates for worms in distilled water, they were very active, which could have caused inaccuracies. To solve the inaccuracies due to their movement, smaller well slides were made. Also, keeping them out of the light source from the microscope may calm them down. In order to expand our knowledge on the effects of alcohol on Lumbriculus variegates, we can increase the number of trials. Other chemicals could be tested on the worms as well. Independent Variable: Alcohol Solution. Dependent Variable: The difference in pulse rate. Control Setup: Distilled water. Constants: Amount of water, temperature, microscope, and time. References: Www.wikipedia.org/wiki/Ethonal#Name Www.newworldencyclopedia.org Www.ableweb.org/volumes/vol-27/07_Bohrer.pdf Materials: 20 worms, 1mM alcohol, 1mM distilled water, Microscope, well slides, cover slides, 4 cups, and dropper.

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