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Sympathetic denervation in the mice ~ My life in San Diego

Sympathetic denervation in the mice ~ My life in San Diego. 生科 04 楊慧儒 891644 2002.12.06. 劉新光 教授 John H. K. Liu, Ph.D. . the regulation of intraocular pressure An elevated intraocular pressure ─ primary open-angle glaucoma

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Sympathetic denervation in the mice ~ My life in San Diego

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  1. Sympathetic denervation in the mice~ My life in San Diego 生科04 楊慧儒 891644 2002.12.06

  2. 劉新光 教授 John H. K. Liu, Ph.D. • the regulation of intraocular pressure • An elevated intraocular pressure ─ primary open-angle glaucoma • the circadian (24-hour) rhythms of intraocular pressure and related ocular functions. • Human study─ eye drop for glaucoma

  3. Summer research • Superior cervical ganglionectomy • Way to measure the pupil size of the mice • Test of eye drop on postsurgical mice

  4. Superior Cervical Ganglion (SCG)

  5. Superior Cervical Ganglion (SCG) • Sympathetic nerve─change in pupillary reflex • Removal or sectioning of this part can reduce the circadian IOP elevation • The outcome of removal of SCG or cutting the cervical sympathetic trunk will mimic human Horner’s syndrome─drug test • PAST~ • Palpebral ptosis and become irreversible from 20th hr • Palpebral retraction

  6. Pharmacology of the sympathetic nervous system hydroxyamphetamine

  7. Drug to Test Horner’s Syndrome

  8. Ganglionectomy Toe & tail Mouse(C57BL/6) Male, 30g, 4 mice Anesthesia (Ketamine+Xylazine) Surgery Fix it on the bench and preparation of instruments

  9. Shave Incision Find SCG Cut SCG (right side) Suture back the fats & the skin Ganglionectomy

  10. Ganglionectomy

  11. Anesthetized Ketamine pentobarbital Conscious Tube Cone Ruler Measurement of pupil size

  12. Eye Drop Test • Solution (saline) • 1 micro-liter, twice, on each eye • Record every 5-10mins • Experimental time: 70mins • M: mouse number • M0: normal mouse • (day): the day after surgery

  13. Result- Hydroxyamphetamine & Tyramine

  14. Result- Cocaine

  15. Result- Epinephrine

  16. Discussion • Compare to rabbits: • Cocaine─ 5~30mins • Hydroxyamphetamine─ 30~45mins • Cocaine, hydroxyamphetamine, and tyramine work on rabbits but not mice. • Only using epinephrine at certain day after surgery(10th-15th days) can have significant result to confirm the success of surgery.

  17. Furthermore • Increase the number of experiment • Find a critical way to measure the pupil size of mice • Electrode stimulation • The mechanism of drug effect

  18. Life in San Diego

  19. Life in San Diego Balboa park

  20. Life in San Diego Mission bay & Balboa park

  21. University of California,San Diego

  22. Campus

  23. Thank you for listening !!!

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