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7-AAD

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7-AAD

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  1. Figure 3. The viability of NICC before and after transfection. The proportion of live cells from NICC before or after transfecttion with control siRNA or TF siRNA was calculated as the percentage of PE annexin V and 7-AAD double negative staining cells. The results are representative of four independent experiments. NICC cultured with the apoptotic agent stauroporine was used as a positive control (stimulated NICC). 5.13% 3.13% 5.89% 105 14.77% 105 104 104 103 103 102 102 79.31% 12.43% 0 0 32.46% 46.88% 105 0 102 103 104 105 0 102 103 104 Supplemental Digital Content 3 Non-Stimulated NICC Stimulated NICC 7-AAD PE-Annexin V TF siRNA transfected NICC Control siRNA transfected NICC Non-transfected NICC 3.18% 4.96% 3.28% 6.54% 2.92% 1.15% 7-AAD 15.92% 9.60% 14.14% 86.33% 76.14% 75.84% PE-Annexin V

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