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Progress Report on tetA Module Transfer and Verification in Dog RBS eCDM8

This report outlines the experimental progress made on October 25, 2013, regarding the tetA module transfer to the psB1A8 backbone, using substances like 1mM Caffeine and 1mM Theophylline. Key steps included iPCR, GGA, and Colony PCR techniques to verify tetA resistance. The initial insert size was approximately 4100 kb, with detailed results associated with multiple steps of the project. The next phases will focus on verifying the insert and completing the program to ensure functional outcomes in the target system.

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Progress Report on tetA Module Transfer and Verification in Dog RBS eCDM8

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Presentation Transcript

  1. Lab Meeting 10/25/2013 Catherine Doyle

  2. TET + 1mMCaffeine TET + 1mM Theophylline TET

  3. c.Dog RBS eCDM8 tetA psB1A8 psB1A2 tetA c.Dog RBS eCDM8 psB1A8

  4. GGA Results MW 1 2 3 4 5 6 7 8 9 10 C1 C2 Insert bp = 4100kb

  5. Steps/Progress • Move tetA module to psb1A8 • iPCR • GGA • Colony PCR • Verify through tetA resistance and PCR

  6. Next Steps • Verify Insert • Finish program

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