1 / 15

Lab Meeting

Lab Meeting. 09/27/13. Program Update. Unafold arrived Need to install g77 complier. Theophylline Detection Results. How much Theophylline do cells need to grow on plates? . Test growth at 0.4, 0.2, 0.1, 0.05, 0.0 mM of Theophylline Look for visible GFP expression. psB1A2. High copy .

ronnie
Télécharger la présentation

Lab Meeting

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Lab Meeting 09/27/13

  2. Program Update • Unafold arrived • Need to install g77 complier

  3. Theophylline Detection Results

  4. How much Theophylline do cells need to grow on plates? • Test growth at 0.4, 0.2, 0.1, 0.05, 0.0 mM of Theophylline • Look for visible GFP expression

  5. psB1A2 High copy Grow o/n @ 37C LB + Amp

  6. Colony streak culture onto plates LB + Amp 0.1mM Theo 100ug/ml Amp 0.05mM Theo 100ug/ml Amp 0.0mM Theo 100ug/ml Amp 0.4mM Theo 100ug/ml Amp 0.2mM Theo 100ug/ml Amp Grow o/n @ 37C

  7. 0.0mM Theophylline 0.05mM Theophylline 0.1mM Theophylline 0.2mM Theophylline 0.4mM Theophylline

  8. How much Theophylline do cells need to grow with a fixed amount of tetA? • Test growth at 0.4, 0.2, 0.1, 0.05, and 0.0 mM of Theophylline • Also, test growth with 10ug/mL of tetA +/- Amp • Looking for visible growth

  9. psB1A2 High copy Grow o/n @ 37C LB + AMP + Theophylline

  10. Colony streak culture onto plates LB + Amp + Theophylline 0.0mM Theo 100ug/ml Amp 10mg/ml tetA 0.1mM Theo 100ug/ml Amp 10mg/ml tetA 0.05mM Theo 100ug/ml Amp 10mg/ml tetA 0.4mM Theo 100ug/ml Amp 10mg/ml tetA 0.2mM Theo 100ug/ml Amp 10mg/ml tetA

  11. Colony streak culture onto plates LB + Amp + Theophylline 0.0mM Theo 10mg/ml tetA 0.1mM Theo 10mg/ml tetA 0.05mM Theo 10mg/ml tetA 0.4mM Theo 10mg/ml tetA 0.2mM Theo 10mg/ml tetA

  12. LB+TetA 0.0mM Theophylline 0.05mM Theophylline 0.1mM Theophylline 0.2mM Theophylline 0.4mM Theophylline

  13. LB+AMP+TetA 0.0mM Theophylline 0.05mM Theophylline 0.1mM Theophylline 0.2mM Theophylline 0.4mM Theophylline

  14. Conclusions • Cell expression of the reporter gene is robust in the presence of the theophylline • Therefore, ecdM8 is likely not being translated • Gst9 cloning a potential solution

  15. Future Work • Finish Program • Develop 10 minute Preview Presentation • Start Evolution Experiment

More Related