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Lab Meeting

Lab Meeting. 10/04/2013. Results – Theophylline Disc. Ecdm8 + Riboswitch + tetA. Ecdm8 + Riboswitch + tetA. Ecdm8 + Riboswitch + tetA. Constructs on same Plasmid. High Promoter. Low Promoter. Results – Caffeine Disc. Ecdm8 + Riboswitch + tetA. Ecdm8 + Riboswitch + tetA.

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Lab Meeting

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  1. Lab Meeting 10/04/2013

  2. Results – Theophylline Disc Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA Constructs on same Plasmid High Promoter Low Promoter

  3. Results – Caffeine Disc Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA Ecdm8 + Riboswitch + tetA Constructs on same Plasmid High Promoter Low Promoter

  4. Results 15 hour absorbance minus initial absorbance with 1 mM Theophylline

  5. Results 15 hour absorbance minus initial absorbance with 5 mM Caffeine

  6. Ecdm8(low copy) + TetA Module(high copy) 0.1mM 0.05mM 0.2mM 0.4mM 0mM of Theophylline

  7. Ecdm8+ TetA Module 0.1mM 0.05mM 0.2mM 0.4mM 0mM of Theophylline

  8. Future work • Test tetA fitness module and ecdm8 with caffeine, 3-methylxanthine, and xanthine • Thesis Preview Tuesday common hour Dana 146

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