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Exploring Stem Cell Differentiation Pathways through Relative Gene Expression Analysis

This supplementary figure presents data on the relative expression levels of key genes - Nanog, c-myc, Klf4, Oct4, Sox2, Mycn, and Arid3b - during differentiation of induced pluripotent stem cells (iPS) to neural stem cells (NSC) and embryonic stem (ES) cells. The study tracks the changes in gene expression at different passages to elucidate the differentiation process. Understanding these pathways can provide insights into cell fate determination and regenerative medicine applications.

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Exploring Stem Cell Differentiation Pathways through Relative Gene Expression Analysis

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  1. Supplementary Figure 1 A B C D 106 0.5 iPS Nanog pmx-c-myc 4 105 piPS 0.4 pmx-Klf4 104 pmx-Oct4 3 Relative expression Relative expression 0.3 pmx-Sox2 Cell number 103 2 0.2 102 1 0.1 101 1 2 3 4 5 NSC piPS iPS ES NSC piPS iPS ES Passage number

  2. Supplementary Figure 1 E F Mycn Arid3b 5kb

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