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Proper Rotor Cleaning Procedures for Spilled Liquids in Cell Culture

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Maintaining rotor cleanliness is essential for safety and equipment longevity. For spilled non-viral cell culture or bacterial media, sanitize wells with 70% EtOH and clean with warm 1% non-alkaline detergent, using a bristled brush if needed. Always rinse with deionized water and dry upside down on a paper towel. For Tritium-contaminated samples, use equal parts of 70% EtOH and 10% SDS. For viral cell culture media, apply an antiviral agent like Enviricide®. Always inspect and maintain rotors to ensure they remain uncontaminated and balanced for all users.

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Proper Rotor Cleaning Procedures for Spilled Liquids in Cell Culture

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  1. Rotor Cleanliness Policy How do I clean the rotor if there is spilled liquids? • Non-viral cell culture or bacterial media • Sanitize wells with 70% EtOH • clean with warm 1% non-alkaline detergent (use a bristled brush if necessary) • rinse with deionized water • dry upside down on a paper towel • Tritium-contaminated samples • Sanitize with equal parts of 70% EtOH and 10% SDS in water • clean as you would for culture/media spills above • Non-hazardous buffer solutions • Rinse wells with deionized water • dry upside down on a paper towel • Viral cell culture media • Sanitize with a antiviral agent such as Enviricide® • clean as you would for culture/media spills above. It is VBI Equipment Resource policy to be good stewards of the rotors by: Removing all rotor well adapters after every run Inspecting the wells for spilled liquids/broken glass Cleaning the rotor wells and adapters if any residual liquids are found NEVER USE BLEACH! Questions? Contact me! Why is this important? Safety… on many levels. And, properly maintaining our equipment so that it will be available for everyone to use for a long time. I recently pulled rotor well adapters out which had pieces of glass and chunks of semi-dried media under it. Anyone using a rotor after you should not have to clean up a mess first. Liquids left in wells can un-balance a rotor. Also, users should not have to be concerned with contaminating their tubes with unknown biological sample left in the well.

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