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Enhancing USFWS eDNA Sampling Capacity for Early Detection of Invasive Species

The USFWS aims to develop long-term eDNA sampling capabilities focusing on southern Lake Michigan and western Lake Erie to enhance early detection of invasive species, specifically Asian carp. Initial resources will support the Fish and Wildlife Conservation Office and La Crosse Fish Health Center in staffing, equipment, and laboratory space for effective eDNA sample processing. Collaborative efforts will refine sampling protocols and establish systematic analysis to decrease turnaround times for results and support invasive species management across the Great Lakes.

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Enhancing USFWS eDNA Sampling Capacity for Early Detection of Invasive Species

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  1. 2.6.2 and 2.6.4 2.6.2: USFWS Capacity for eDNA Sampling for Early Detection 2.6.4: USFWS Capacity for eDNA Sample Processing Mike Weimer

  2. Introduction and Objectives 2.6.2: Will provide USFWS Fish and Wildlife Conservation Office facilities the initial resources to conduct integrated long‐term early detection activities with a particular focus on locations in southern Lake Michigan, western Lake Erie and other invasional hotspots. 2.6.4: Will provide USFWS La Crosse Fish Health Center with initial resources to develop adequate staffing, equipment, and laboratory space to begin processing eDNA samples.

  3. Current Knowledge • FY 2011 Action: • Task 2.6.2: • Testing and refinement of capabilities for monitoring and sampling eDNA outside the CAWS • Development of an eDNA sampling protocol that will be incorporated into long term monitoring strategy in collaboration with participating agencies.

  4. Current Knowledge (continued) • FY 2011 Action: • Task 2.6.4: • Development of the capacity for the systematic analysis of eDNA samples - initially from the CAWS and then other locations around the Great Lakes • Assist collaborating agencies in validation of eDNA calibration and sample processing as facility comes on line decreasing turn‐around time for results • Asian carp highest priority for sample processing followed by other high risk AIS

  5. Future Plans 2.6.2 • Fully develop the capacity for implementing an eDNA sampling program at USFWS Great Lakes Fish and Wildlife Conservation Offices. Q4 - 2012 • Support the establishment of a biologically based statistically tenable eDNA sampling protocol for use basin wide. Q4 - 2011 • Implement an eDNA draft sampling protocol at other areas of concern with focus on southern Lake Michigan, western Lake Erie and other potential hotspots. Q4 - 2012

  6. Future Plans 2.6.4 • Meet with USACOE ERDC personnel to establish timeline and feasibility of eDNA sampling technology transfer. Q2 - 2011 and ongoing • Develop guidelines and process for eDNA technology transfer. Q2/Q3 - 2011 and ongoing • Establish new lease agreement with GSA allowing for remodel and expansion of the La Crosse FHC facility. Q3 - 2011.

  7. Future Plans 2.6.4 (continued) • Remodel and expand La Crosse FHC facility, purchase needed equipment and supplies. Q3 - 2012 • Hire appropriate personnel. Q4 - 2011.

  8. Technological or Other Hurdles • 2.6.2: • Weather and access to sampling sites • Quality Assurance/Quality Control (QAQC) process for sample contamination • Calibration of eDNA technology is needed • 2.6.4: • Calibration results/lack of results may influence timeline for transfer to USFWS • Weather may influence construction timelines

  9. Data Gaps • Calibration work will be key to quantify what an eDNA detection specifically means • Marker development may not cover all target species • La Crosse initial sample processing capacity will be 50 samples per week which is less than is currently being processed

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