HEMATOLOGIC INVESTIGATION. CLINICAL LABORATORY DIAGNOSTICS OF ANEMIAS

1. HEMATOLOGIC INVESTIGATION. CLINICAL LABORATORY DIAGNOSTICS OF ANEMIAS

2. Anemia- decrease in level of hemoglobin and red blood cell (RBC) mass. • Anemic syndrome- is combination of laboratory signs of an anemia and its clinical manifestations caused by a hypoxia and a hypoxemia

3. Anemic syndrome Complaints • General: weaknesses, fatigue, dizziness, syncopal and orthostatic condition, decrease of memory. • From the cardiovascular system: a dyspnoe, palpitation; in case of presence coronary artery disease - worsening of its symtoms.

4. Anemic syndrome Physical examination • at external medical examination - pallor; • from the cardiovascular system: a tachycardia, systolic murmur above an apex cordis

5. Laboratory methods The World Health Organization's criterion for anemia in adults is Hb values less than 12.5 g/dL. Usually, US values are slightly higher. Anemia is suggested in males with Hb levels less than 13.5 g/dL and in females with Hb levels less than 12.5 g/dL.

6. Classification of anemia І. Pathogenetic: 1. As a result of loss of erythrocytes during the bleeding (acute and chronic posthemorrhagic anemia); 2. As a result of increase destruction of erythrocytes (hemolytic anemia); 3. As a result of the broken synthesis of hemoglobin (iron-deficiency) and maturing of erythrocytes (megaloblastic and aplastic anemia).

7. Classification of anemia II. By a degree of severity: 1. Easy - decrease Hb on 5-19,9 % from initial 2. Average - on 20 -39,9 % 3. Heavy - more than on 40 % The decrease Hb up to 5 % is considered as norm

8. III. On ability to regeneration:1.Normoregenerative - reticulocytes 0,2-1,2 %2.Hyporegenerative - reticulocytes < 0,2 %3.Hyperregenerative - reticulocytes > 0,2 %

9. IV. Under the contents of a hemoglobin in erythrocytes:1. Hypochromic (mean corpuscular hemoglobin concentration (MCHC) < 32 g/dL) - at iron-deficiency

10. 2.Hyperchromic (MCHC > 36 g/dL) - В12-deficiency anemia

11. 3. Normochromic (MCHC 32-36 g/dL) - majority of other anemia

12. V. On a diameter of erythrocytes:1. Normocytic (mean corpuscular volume (MCV) -84-96 fL)

13. 2. Microcytic (MCV < 84 fL) - iron-deficiency, hemolytic anaemia

14. 3. Macrocytic ( MCV > 96 fL) - В12-deficiency

15. Sideropenic syndromes • From the skin covers, appendages of a skin and mucous membranes: • dryness of a skin, • fragility of a hair fragility and banding nails, they become flat, sometimes accept the concave form (koilonychias);

16. Sideropenic syndromes 2. From the gastrointestinal tract: • decrease and distortion of appetite, • dysphagy, • constipations or diarrhea, • glossitis, burning of tongue, • esophagitis;

17. Sideropenic syndromes • unusual predilections for some smells - acetone, petrol; • angular stomatitis; • the distortion of taste as desire is a chalk, tooth paste, ashes, paint; • abnormality of a swallowing with sensation of an alien body in a throat.

18. Causes of development of iron-deficiency states: • Blood loss • Vegetarians • Prolonged achlorhydria • Extensive surgical removal of the proximal small bowel or chronic diseases, such as pancreatitis, enteritis. • Increased iron requirement: From birth to age 2 and during adolescence During pregnancyand lactation

19. Lab StudiesI. In chronic iron deficiency anemia, the cellular indices shows a microcytic and hypochromic erythropoiesis

20. Lab Studies • Decrease of iron concentration. Normal serum iron is 13 to 27 μmol/L for men and 11 to 25 μmol/L for women. • Increase of iron-binding capacity. Total iron-binding capacity is 45 to 81 μmol/L. • Decrease of serum ferritin. The range of normal in most laboratories is 30 to 300 ng/m.

21. Lab Studies Decrease of sideroblasts in the bone marrow aspirate. Normally, ther are 20 to 40% sideroblasts.

22. Megaloblasticanemia • The molecular basis for megaloblastosis is a failure in the synthesis and assembly of DNA. • The most common causes of megaloblastosis are cobalamin and folate deficiencies. • Prevalence of megaloblastosis is enlarged with the years and makes up at young persons about 0,1 %, at elderly up to 1 %. Women are sick more often.

23. Pathophysiology • Dietary intake (meat, liver, kidneys, eggs, milk, cheese, beans) is the source of cobalamin and folate because humans cannot synthesize these substances. • Cobalamin must be bound to intrinsic factor, and this complex is taken up in the terminal ileum.

24. Pathophysiology • Cobalamin enzymes - metabolism of nucleic acids, and at its deficiency mitotic processes in fast-growing tissues (a bone marrow, an epithelium of a GI tract) are broken, - exchange of fat acids, synthesis and neogeneses of a myelin, and at its disadvantage these processes are broken. Also a defect in adenosyl-cobalamin-dependent conversion of methylmalonyl coenzyme A to succinyl coenzyme A exists.

25. Causes ofcobalamin deficiency • Nutritional deficiency • Food-cobalaminmalabsorption (gastric anacidity) • Absence of functional intrinsic factor • Severe abnormalities in the terminal ileum due to ileal resection, regional ileitis, or lymphoma • Helminthic invasion • Blind loop syndrome • Drugs • Cytostatics • Anticonvulsants • Oral contraceptives • Nonsteroidantiinflamatory drugs • Some antidiabetic drugs • Colchicine

26. Clinical features • Anemic syndrome • Jaundice • Gastrointestinal manifestations: anorexia; diarrhea;

27. Clinical features sore tongue, which on inspection will be smooth and beefy red

28. Clinical features 4.Neurologic manifestations: numbness and paresthesia in the extremities; weakness; ataxia; sphincter disturbances; diminished or increasedof reflexes; positive the Romberg and Babinski’s signs; diminish position and vibration senses; disturbances of mentation

29. Lab Studies • macrocytosis • fullyhemoglobinizederythrocytes • neutrophils show hypersegmentation of the nucleus • leukocyte and platelet count may be decreased

30. Lab Studies Nucleated RBC

31. Lab Studies • Decrease serumcobalamin level. The normal range is 300 to 900 pg/ml. • Bone marrow aspirate is hypercellular with a decreased myeloid/erythroid ratio. Megaloblastic RBC precursors are abnormally large and have nuclei that appear much less mature than would be expected from the development of the cytoplasm (nuclear-cytoplasmic asynchrony). The nuclear chromatin is more dispersed than expected, and it condenses in a peculiar fenestrated pattern. Abnormal mitoses may be seen.

32. Lab Studies

33. Lab Studies • Increase in unconjugated bilirubin and lactic acid dehydrogenase in plasma • Abdominal x-ray films, upper and lower GI series, and CT scans

34. HemolyticAnemias • group of the diseases are characterized by raised intracorpuscular (in cells of cytophagous system) or extracorpuscular (in a vascular channel) destruction (hemolysis) of erythrocytes and decrease of duration their life (less than 120 days).

35. Classification

36. Clinical and laboratory signs of hemolysis • an anemic syndrome with a normochromal anemia; • a reticulocytosis; • a jaundice of a skin and mucosae; • hepato- and splenomegaly; • augmentation of the urobilinogen content in urine; • augmentation of the stercobilinogen content in a feces; • level of unconjugated bilirubin elevates; • low or absent levels of serum haptoglobin; • rising of a level serum iron; • the life span of RBC may be shortened; • erythroid hyperplasia of the bone marrow.

37. Acquired hemolytic anemia

38. Causes of immunohemolytic anemia

39. Clinical manifistations • anemia, • spherocytosis, • splenomegaly, • venous thrombosis, • positive direct Coombs test

40. Aplastic anemia • is a bone marrow failure syndrome characterized by peripheral pancytopenia and marrow hypoplasia.

41. Epidemiology • TheincidenceofacquiredaplasticanemiainEuropeandIsraelis 2 casespermillionpersonsannually. • InThailandandChina, ratesof 5 to 7 permillionhavebeenestablished. • Ingeneral,menandwomenareaffectedwithequalfrequency, butthereis a biphasicagedistribution, withthemajorpeakintheteensandtwenties and a secondriseintheelderly.

42. Classification and etiology

43. Drugs and chemicals associaed with aplastic anemia

44. Clinical manifestations • Anemic syndrome • Hemorrhagic syndrome - bleedings (nasal, uterine, gastrointestinal), ecchymoses and petechias • Syndrome of infectious complications.

45. Lab studies • A paucity of platelets, red blood cells, granulocytes, monocytes, and reticulocytes • Bone marrow aspirate from iliac crest - mainly fat under the microscope, with hematopoietic cells occupying less then 25% of the marrow space

46. Bone marrow aspirate from iliac crest Normal Aplastic anemia

47. Hemorrhagic syndrome Hemorrhagic spots on surface of the skin, patechiae, purpura, heleangiectasis, and hemorrhages. These signs are present not only on the skin, but in subcutaneous fat, muscle, brain too. The hemorrhagic syndrome is usual in case of inherited thrombocytopenic purpuras, neonatal thrombocytopenic purpuras. The other signs of this syndrome are bleeding, hematuria, upper and lower gastrointestinal tract hemorrhage, prolonged bleeding from the umbilical stump or from veni-punctures, intracranial hemorrhage.