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Undergrad Research Symposium Meeting and Lab Protocol Reminders

Join us for an important meeting next week as we prepare for the Undergrad Research Symposium. A reminder for the meeting with professors on Friday the 16th from 5-6:30 PM is also included. Don't miss our Movie Night on Thursday featuring "Real Genius" and "Caprica"—bring your suggestions! Additionally, please make note of the safety meetings on the 10th and 17th, and review lab protocols to ensure a professional lab environment. Remember to keep the lab clean and maintain detailed notebooks.

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Undergrad Research Symposium Meeting and Lab Protocol Reminders

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Presentation Transcript

  1. Buenos Dias! • Meeting time for next week? • Undergrad Research Symposium • Reminder – meeting with professors Friday the 16th from 5-6:30. • Movie Night on Thursday • Real Genius/Caprica – other suggestions? • New Location • Safety Exams!? • Safety Meetings on the 10th and 17th • 9-12 or 10-1?? • Check out these two Generally Informative Syn Bio Documents. • Nice Job at EOH (Pictures!) • Projects can be classified as dealing with a specific component of a system: inputs, information processing, or outputs.

  2. Lab Protocols

  3. General Lab Basics • While in lab remember act professional • Just be courteous • Keep a great lab notebook • Always write everything down every time • All information will be in duplicates (triplicates with the wiki) • One for the in-lab notebook; One for your notebook; (And one for the wiki) • Try to keep lab space as clean as possible • Dishwashing, autoclaving and disinfecting will be covered a Saturday

  4. Bacterial Growth • About 2-4 hours Log phase begins • 8-10 Stationary phase begins • 12 hours = Overnight (middle of Stationary phase) • 24+ hours es no bueno… • We use LB liquid and solid media to grow cells • 10 grams Tryptone • 5-10 grams NaCl • 5 grams Yeast Extract • 15 grams agar (solid media) • all in a 1L dH2O batch

  5. Cloning • Biobricks http://ginkgobioworks.com/support/ • Digest • Run a gel • Ligate • Test resistance • From the ‘some • PCR • Design primers • Ligate • Test resistance • Analyze <--TRANSFORM

  6. Cloning • Digestions/Ligations

  7. Cloning • Transformation • Need competent cells • Done by either: • Heat Shock Transformation using CaCl, • Wash cells with CaCl solution reagent • Relatively inexpensive • High Efficiency Electroporation • Shorter Procedure • Need expensive cuvettes • Extremely efficient • Wash cells with 10% Glycerol

  8. Cloning • Gel Electrophoresis • ALWAYS USE LADDERS!!!!

  9. References • Current Protocols in Molecular Biology • Search in Pubmed

  10. Stuff • Plate Reader • basic info about designing primers • how a pcr works. • Stuff about mini/midi preps. • Also, how to streak a plate, how much culture and broth to use for a liquid suspension. • How long is stuff good for in the 4deg. room. what do you keep in the -20 vs. -80 and cryostocking stuff. • How to do ligations and digestions • When to autoclave • pHing stuff – when and how • Nano-drop (it like it’s hot) • List of Supplies

  11. Questions!? • What procedure type stuff did we forget? What do you guys know and consider important? What questions do you have? • Questions about the regional conference.

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