1. PRODUCTION OF HIGHLY PURIFIED BOVINE TRANSFERRINGroup 5BArjun Goel, Connie Marcelli, Lianne Kark, Sara Dang, Vivien TaiFaculty AdvisorProf C.A. Mims

2. Timetable

3. Agenda Mass Balances & Waste Treatment Key Unit Discussion Unit Specifications Current Challenges

4. Process Overview Crude Paste Filtration Chromatography Dry Transferrin Powder

5. Agenda Mass Balances & Waste Treatment Key Unit Discussion Unit Specifications Current Challenges

6. Mass Balance — Feeds Starting Paste 18 kg Distilled Water 160 L FeCl3, NaHCO3, Na-Citrate for iron Binding Celite 6 kg D.I. Water 571 L Process EDTA for Iron Chelating Diafiltration Buffers Wash for Column

7. Mass Balance — Products Solids Liquids Immunoglobins for recovery Trace Proteins Water 740 L Inorganics FeCl3, NaHCO3, Na-Citrate, EDTA, NaOH Process Ethanol 4.5 kg Celite 6 kg Purified Apotransferrin 350g / Batch

8. Option 1 Option 2 Waste Water Solid Waste Reverse Osmosis Process Waste Water Ion Exchange Acid/Base Addition Neutralize To Sewer Waste Treatment--Liquids

9. Waste Treatment — Solids • Reverse Osmosis cartridges • Filter press cakes Disposal via contractor • Mainly salts and celite trapped in filters

10. Agenda Mass Balances & Waste Treatment Key Unit Discussion Unit Specifications Current Challenges

11. Key Unit: R-101

12. Key Unit: R-101 • Stirred Tank • Cylindrical • Conical Bottom • Tank Material: SS316 • In Cold Room 0-5 oC

13. Key Unit: R-101 • Volume of solution in tank: 400 L • Unit Selected: • Capacity: 757 L ( 200 Gal ) • Depth: 1.46 m • Diameter: 0.91m

14. Key Unit Accessories • Level Indicator • pH Monitor • Temperature Indicator • SS316 piping • Valve • Air Diaphragm Pump • Mixer

15. Agenda Mass Balances & Waste Treatment Key Unit Discussion Unit Specifications Current Challenges

16. Filter Press: FP-101 • 12” in diameter • Stainless Steel plates and frames • Cellulose Pads • Four 1” sludge frames • 40 psi • Cost: $6 703

17. Filter Press: FP-101 3 Applications: • 4 Plates: removes 12 000 g paste @ 19 L/min 2) 2 Plates: removes 1 320 g denatured protein @ 9 L/min 3) 2 Plates: collects 3 600 g precipitated protein @ 9 L/min

18. PrefiltrationSF-101 • Before ultrafiltration • Millipore low protein binding cartridge filter • Filtration Area: 0.7m2 • 80 psi • Entrains particles > 0.5 mm • US$208 for 2

19. Ultrafiltration

20. Spiral Wound FilterUF-101 • Concentration: 450 L to 90 L • Polysulfone • Flow: 100 L/hr • Flux: 22 L/m2 hr • Pressure: 60 PSI • Cost: $8 212

21. Spiral Wound FilterUF-102 • Concentrate from 30L to 15L • Regenerated cellulose Membrane • Pressure: 60 PSI • Flow: 10L/hr • Flux: 11 L/m2hr • Cost: $2 154

22. Hollow FiberUF-201 • Concentrate from 12 L to 6 L • Polysulfone • 25 PSI • 6 L/hr • 30 L/ m2hr • Peristaltic Pump

23. DEAE Column

24. DEAE Sepharose Fast Flow Column: C-201 • Automated • Includes: The Column The Bioprocess Skid The computer DEAE Resin • Total Cost: CND$211, 000

25. Water Distillation ColumnT-204 • Rate: 190 L/24 hrs • Storage capacity: 280 L • Size: 2m x 1m x 0.6m • Cost : US$ 3 945

26. Deionized Water UnitI-101 • Flow range: 15 – 23 L/min • Size: 1.5m x 0.6m x 0.4m • Cost: US$ 4 142

27. Similar unit used in plant Capacity: 8-12 L ~6 L of water to condense per batch LyophilizerL-101

28. Future Directions • Key unit development • P&ID • Design equations • Control Philosophy • Economic Evaluation • Plant Layout • Appendices