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بسم الله الرحمن الرحيم Palestine Polytechnic University Engineering & Technology Collage

بسم الله الرحمن الرحيم Palestine Polytechnic University Engineering & Technology Collage Electrical &Computer Department Biomedical Engineering Program Biomedical Eng Lab (II) Chemistry Analyzer (Spectrophotometer Device) Eng.Fidaa Jaafreah. Chemistry Analyzer (Spectrophotometer Device).

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بسم الله الرحمن الرحيم Palestine Polytechnic University Engineering & Technology Collage

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  1. بسم الله الرحمن الرحيم Palestine Polytechnic University Engineering & Technology Collage Electrical &Computer Department Biomedical Engineering Program Biomedical Eng Lab (II) Chemistry Analyzer (Spectrophotometer Device) Eng.Fidaa Jaafreah

  2. Chemistry Analyzer (Spectrophotometer Device) • Spectrophotometer : is a photometer device for • measuring light intensity that can measure • intensity as a function of the color or more • specifically the wavelength of light .

  3. Tests Type Used By Spectrophotometer • (الكبد)1- Liver Function Test (LFT),GPT,GOT • (الكلى) 2- BUN ,Creat • (فحص الصفري) 3-Albumin • (السكري) 4-FBS, RBS

  4. Table 3.1 Normal and toxic levels of various molecules in the body given in both US and European (SI) units (Pagana and Pagana, 1995). NA denotes “not available”.

  5. Spectrophotometer Types • 1- Single Beam • 2- Double Beam

  6. Single Beam Spectrophotometer

  7. Double Beam Spectrophotometer

  8. Spectrophotometer Block Diagram

  9. Calibration Method

  10. Calibration Steps 1. Turn on your instrument and allow it to warm up for 20 minutes. 2. Select the %Transmittance operating mode by pressing the MODE key. 3. Set the wavelength to 519nm. 4. Insert the cuvette filled with distilled water in the well of the cuvette holder. 5. Pressing the 100%T/0-Abs key until display read 100.0%T or 0.000A, then remove the cuvette with water.

  11. Troubleshooting

  12. Power Supply Circuit for Spectrophotometer

  13. Detector Circuit

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