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Blake Hovde University of Washington, Department of Genome Sciences, Biology

Genomics of the haptophyte Chrysochromulina tobin : Cell Division, Lipid Production and Storage in an Oil Producing Alga. Blake Hovde University of Washington, Department of Genome Sciences, Biology Rose Ann Cattolico Lab. Do we need to study new algal species ?. The benefit is two fold:

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Blake Hovde University of Washington, Department of Genome Sciences, Biology

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  1. Genomics of the haptophyte Chrysochromulina tobin: Cell Division, Lipid Production and Storage in an Oil Producing Alga Blake Hovde University of Washington, Department of Genome Sciences, Biology Rose Ann Cattolico Lab

  2. Do we need to study new algal species? The benefit is two fold: • New models to study desirable algal traits -biological/metabolic • More desirable traits available -better starting point for manipulation

  3. Plummeting sequencing costs provide great opportunity

  4. Sequencing projects on algal species are increasing in number Keeling et al. MMETSP project PLOS Bio 2014

  5. Chrysochromulinatobin Deodato, Barlow, Hovde et. al. (in final preparation)

  6. Chrysochromulina tobin as a model organism 24 hours: 12 hours dark / 12 hours light Lipid Accumulation Sexual Reproduction? --Likely, and is ideal for genetics Lipid Depletion

  7. Isolation of C. tobin Reiterative flow cytometry Environmental Sample Lab conditions Antibiotic treatments Sequencing Cleaned Culture

  8. Sequencing and gene annotation pipeline High throughput sequencing (Illumina and 454) Blast2GO Assembly “Omes” competed: • Genomes: • Mitochondrial • Chloroplast • Nuclear • Transcriptomes: • 7 time points over photoperiod • Proteomes • Whole cell • Lipid body isolation Hovde et. al. (BMC Genomics 2014), Hovde et. al. (in final preparation), Brunelle et. al. (in prep) KEGG database

  9. Lipid pathway from Radakovitset. al. 2010

  10. Dependable fatty acid content of C. tobin • X ug/L production • Proposed standard for fatty acid content • DHA • EPA • Stearic • Palmitic • Myristic • …etc… Total lipid ~17mg/L Bigelow et. al. (Algal Research, 2013)

  11. Mixotrophyand defense systems for algal biomass production • Fatty acid productivity is increased when grown w/ bacteria • Genomic identification of multiple defense/mixotrophic mechanisms • Useful for large pond cultures • Potential for engineering/culture maintenance Hovde et. al. (in final preparation)

  12. Conclusions • New algal model species are easier and cheaper than ever before to develop • Flow cytometery, antibiotics • ‘Omics’ costs / informatics • New species can provide new metabolic pathway insights • Co-culturing provides opportunities for higher productivity in C. tobin • New species provide mor”e parts” for engineering efforts • Current generation genome editing tools are powerful

  13. Acknowledgements • Cattolico Lab (UW) • Rose Ann Cattolico • Chloe Deodato • Heather Huntsperger • Will Yost • John Patterson • Stephanie Brunelle • Bill Hardin • RocapLab (UW) • Gabrielle Rocap • Cedar McKay • MonnatLab (UW) • Ray Monnat • Ryan Sinit • Pacific Northwest Natl. Lab • Mary Lipton • Los Alamos Natl Lab • Shannon Johnson • Shawn Starkenburg Funding and support:

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