1 / 7

Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs

Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Rui Yi, Yi Qin, Ian G. Macara and Bryan R. Cullen Genes & Development (2003) 17:3011-3016 Wan-Ting Chen. Introduction. MicroRNAs (miRNAs): ~22nt noncoding RNAs observed in a wide range of eukaryotes

hateya
Télécharger la présentation

Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs Rui Yi, Yi Qin, Ian G. Macara and Bryan R. Cullen Genes & Development (2003) 17:3011-3016 Wan-Ting Chen

  2. Introduction • MicroRNAs (miRNAs):~22nt noncoding RNAs observed in a wide range of eukaryotes • Small interference RNAs (siRNAs):endogenous or synthetic short ds RNAs~22nt • Short hairpin RNAs (shRNAs): artificial generated and usally vector-encoded, resembles pre-miRNA in structure • Exportin-5 (Exp5):belongs to karyopherin family, mediates the nuclear export of adenovirus VA1(highly structured 160nt noncoding RNA) Exportin 5?

  3. Materials - plasmids & siRNAs miR-30binds and inhibits the luciferase expression pri-miRNAof miR-30 pre-miRNA of miR-30 ~22nt synthetic miR-30 RNA duplex

  4. Exp5 siRNA inhibits pre-miRNA & shRNA to silence target mRNA • Loss of Exportin-5 expression specifically relieves inhibition of gene expression caused by pre-miRNA or shRNA pri-miRNA shRNA pre-miRNA Synthetic RNA duplex siRNA

  5. Exp5 siRNA results in a decrease in the mature miRNA expression • No accumulation of pre-miRNA intermediate? Analysis of miR-30 RNA expression

  6. Exp5 binds pre-miRNA in the presence of Ran-GTP • Exportin-5 binds pre-miRNA in a Ran-GTP dependent manner Gel-shift analysis

  7. Conclusion and Significance • Exportin-5 is required for nuclear export and biological activity of pre-miRNA and shRNAs, but not for synthetic siRNA function • Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of Ran-GTP • This study identify a major cellular export cargo for Exportin-5 and define a novel cofactor for miRNA biogenesis and function

More Related