1 / 11

Review

Review. Lab Exercises 8-11. Exercise 8. What is the purpose of this exercise? What is a capsule? What is the capsule made of? What is the importance of the capsule? Describe the staining procedure for staining the capsule What bacteria we use for the capsule stain procedure?.

Télécharger la présentation

Review

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Review Lab Exercises 8-11

  2. Exercise 8 • What is the purpose of this exercise? • What is a capsule? • What is the capsule made of? • What is the importance of the capsule? • Describe the staining procedure for staining the capsule • What bacteria we use for the capsule stain procedure?

  3. Exercise 8 cont. • What is a flagella? • What is taxis? • Why do we stain the flagella? • What bacteria we observed the flagella? • What is an endospore? • Why do bacteria produce spores? • Describe the composition of the endospore

  4. Exercise 8 cont. • When do endospores dissolve? • Describe the procedure to stain endospores • What bacteria we use for the endospore stain procedure? • What is mycolic acid? • What antibiotics are used against TB? • Describe the procedure for acid fast stain • What bacterias we use for this staining procedure?

  5. Exercise 9 • What is the purpose of the exercise? • Explain the major steps involved in obtaining pure cultures from a mixed population • Explain the difference between selective and differential media • Describe the appearance of the following bacteria in EMB • E. coli, Enterobacter, Klebsiella, Proteus, Salmonella, Shigella • Explain the color changes on EMB

  6. Exercise 9 cont. • Describe the growth of the following bacterias on TSA • M. luteus, S. epiderdimis, E. coli, E. aerogenes • Describe the growth of the following bacterias on EMB • M. luteus, S. epiderdimis, E. coli, E. aerogenes • Describe the growth of the following bacterias on CNA • M. luteus, S. epiderdimis, E. coli, E. aerogenes

  7. Exercise 10 • What is the purpose of this exercise? • Explain the composition of the blood agar? • Differentiate between beta, alpha, and gamma hemolysis on blood agar • Describe the composition and use of Mannitol Salt Agar (MSA) • Explain the catalase test

  8. Exercise 10 cont. • Explain the coagulase test • Explain the bacitracin or Taxo A disc test • Describe the composition and use of the Bile Esculin Agar • Explain the 6.5% NaCl testing • Explain the Optochin or Taxo P disc test

  9. Exercise 10 cont. • Describe the growth on blood Agar of: • S. aureus, S. epidermidis, E. faecalis, S. bovis • Describe the growth on MSA of: • St. aureus, S. epidermidis, E. coli • Describe the coagulase test on S. aureus and S. epidermidis • Describe the growth of E. faecalis and S. bovis on Bile Esculin Agar

  10. Exercise 10 cont. • Describe the growth of S. bovis and E. faecalis on 6.5% NaCl • Describe the growth of S. pyogenes with Taxo A • Describe the growth of S. pneumoniae with Taxo P

  11. Exercise 11 • What is the purpose of the exercise? • How do you differentiate Staphylococcus and Streptococcus. • How do you know if you have S. pyogenes in your throat?

More Related