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In search for whitefly attractants

In search for whitefly attractants. Willem Jan de Kogel, Chamila Darshanee Lunuwila, Rob van Tol, Antje de Bruin & Gerrie Wiegers. Bemisia tabaci. Occur in Europe Whiteflies from non-European origin may contain q-viruses Therefore introduction of non-European B. Tabaci not allowed

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In search for whitefly attractants

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  1. In search for whitefly attractants Willem Jan de Kogel, Chamila Darshanee Lunuwila, Rob van Tol, Antje de Bruin & Gerrie Wiegers

  2. Bemisia tabaci • Occur in Europe • Whiteflies from non-European origin may contain q-viruses • Therefore introduction of non-European B. Tabaci not allowed • WP4: improve detection of whiteflies • WP7: detect q-virus in whiteflies

  3. Aim • Find whitefly attractants for improved detection/monitoring/control • Find tomato cultivars that are more attractive then others • Check if whitefly – tomato plant interaction affects attractivity • Analyse chemical compounds involved in attraction • Develop attractants in practical tools

  4. Y-tube Olfactometer Air Charcoal Filter Charcoal Filter Sucking pump Y-Tube each arm 5cm Flow meter Wind speed = 10cm/S Temperature = 21-25 0C

  5. Olfactometer: preference for Santa Clara over Money maker (small difference)

  6. Infested Uninfested Olfactometer: Male whiteflies prefer infested tomato “Santa Clara” plants over uninfested plants Santa Clara Money Maker

  7. Wind Tunnel Experiments

  8. Windtunnel: whitefly infestation increases attractiveness of Santa Clara.

  9. Windtunnel: whitefly infestation more attractive than mechanical damage

  10. Windtunnel: Infested Santa Clara preferred over infested Moneymaker

  11. Greenhouse trial: result confirmed

  12. Greenhouse trial: result confirmed

  13. Conclusion: • Tomato cultivars differ in attractivity to Bemisia tabaci • Previous infestation increases attractivity strongly • Results y-tube, windtunnel greenhouse consistent • Attractivity (at least partly) due to plant odour

  14. Follow-up: • Follow-up: option to continue topic as PhD-project: • Publish results • Identify volatile compounds by headspace collection of infested vs control Santa Clara plants followed by GC-MS • Test potentially interesting compounds with bio-assays • Test attractants in combination with yellow sticky traps in greenhouse

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