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Instrument Performance --Tests

Instrument Performance --Tests. Field illumination (plastic slides, beads) Axial resolution (mirror) Chromatic aberration (1 u beads, mirror) Laser power (meter, test sample) Laser stability (plastic slide, transmission detector) Beam alignment (focal check,mirror,bead). Deviation + 8%.

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Instrument Performance --Tests

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  1. Instrument Performance --Tests • Field illumination (plastic slides, beads) • Axial resolution (mirror) • Chromatic aberration (1 u beads, mirror) • Laser power (meter, test sample) • Laser stability (plastic slide, transmission detector) • Beam alignment (focal check,mirror,bead)

  2. Deviation + 8%

  3. Deviation + 4%

  4. Deviation + 17%

  5. 647 488 568 Deviation = +10%, +17%, +13%

  6. UV Field Illumination 12.5% intervals 47 123 INTENSITY=230 100X Objective (1.4 NA)

  7. 5X Objective (.25 NA) 12.5% intervals

  8. 20X Objective (.7 NA) 12.5% intervals

  9. Axial Registration Mirror Test 63X objective (1.2NA) (Chromatic Aberration)

  10. 63x ( 1.2NA) objective 647 568 488

  11. 568 647 488

  12. Bad Galvanometer

  13. 488 nm + 568nm 568 nm 488 nm + 568nm 568 nm

  14. Test Samples • Beads: Spherotech 10 micron, MP focal check, 1 micron, submicron • Front silvered mirror • Silicon chip • Biological samples-Diatoms, Pollen, MP Fluo slide, User defined specimen • Fluorescence Plastic Slide

  15. A B C C F E D

  16. Dry Objectives • Lower NA’s --Less resolution • Longer working distance • Cleaner working environment

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