1 / 18

Sampling For Success

Sample collection & submission, chain of custody, choosing the right test for Microbiological testing. Sampling For Success. Common Types of Microbiological Samples for Food Plants. Swabs (stick, sponge, cloth) Bulk – Raw materials, Finished Products Water.

hollie
Télécharger la présentation

Sampling For Success

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Sample collection & submission, chain of custody, choosing the right test for Microbiological testing Sampling For Success

  2. Common Types of Microbiological Samples for Food Plants • Swabs (stick, sponge, cloth) • Bulk – Raw materials, Finished Products • Water

  3. Preparing to Collect Swab Samples • Appropriate type and amount of swabs • Labelling materials • Sanitizer • Neutralizing buffer • Chain of custody • Sampling plan

  4. Types of Swabs • Stick • Sponge • Cloth

  5. Swabbing Technique • Label swab, noting sampling location • Open swab aseptically and swab sample site • Close swab and sanitize swab site • Refrigerate swabs until transport to laboratory

  6. Preparing to Collect Product Samples • Sterile Sampling Materials • Spoons, forceps, knives • Sample bags or containers • Gloves, other protective apparel as required • Labelling Materials • Chain of Custody • Sampling Plan

  7. Product Sampling Guidelines • Collect unopened containers if possible • If collecting from bulk containers, ensure sample is representative of the product being sampled • Make sure to collect at least enough sample to meet analysis requirements • Store samples at appropriate temperature in preparation for delivery to the laboratory

  8. Planning for Collecting a Water Sample: • Sterile Sample Bottles • preservative • Labels • Chain of Custody • Cooler and Ice Packs • Transportation Arrangements

  9. Water Sample Site Considerations Sites to Avoid: • Dead end mains • Seldom used faucets • Faucets with a point of use filter eg. Carbon • Garden or other flexible hoses

  10. Collecting the Water Sample • Pre-label the sample container -Date, time of sample, site location • Remove any aerators or screens from the faucet • Run the faucet cold for at least 2 minutes • Open the sample bottle and collect the water until the shoulder, leaving a headspace • Seal the sampling container tightly

  11. Preparing Samples for Shipping to the Laboratory • Place samples in a cooler with an ice pack • Do not allow water samples to freeze • Avoid loose ice • Include Complete Chain of Custody (COC) • Label cooler clearly with site location and destination laboratory location

  12. Important Components of a Chain of Custody (COC) • Identifies sender and receiver of sampled materials • Specifies analyses and methodologies to be performed on samples • Provides descriptions of samples for reporting of analysis results • Serves as a legal document for the traceability of the handling and possession of samples

  13. Points to Remember when Completing COCs • Contact information for company and sampler is available • Sample descriptions allow for the easy identification of the samples included • Analysis and methodology indicated • Sampling plan requirements clearly stated

  14. Chain of Custody (Water) • Client Information • Sample Site Identifications • Name of Sampler • Date and time of sample collections • Analysis Requested • Drinking/ Non-drinking water

  15. Choosing the Right Test • Consider the Objectives of Testing: • Sanitation monitoring • Standard Plate Count, Coliforms, Yeast & Mold • Regulatory compliance • Correct method for compliance • Lab accreditation for methodology • Pathogen screening • Appropriate for the product/environment

  16. Importance of Proper Sampling • From J. Moldenauer and Sutton, “Towards an Improved Sterility Test,” Commentary, PDA Journal of Pharmaceutical Science and Technology Odds of catching sterility breakdown in a batch when taking 20 samples for USP sterility test

  17. Thank you Contact: Alex Montgomery IG MicroMed Environmental Inc. 604-279-0666 alex@igmicromed.com

More Related