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Mechatronics Project EMMET System ElectroMechanical Membrane-Enabled Transfer System

Mechatronics Project EMMET System ElectroMechanical Membrane-Enabled Transfer System Carlo Yuvienco Keeshan Williams Alberto Giacomello. C. Yuvienco; K. Williams; A. Giacomello EMMET System. Objective : Protein Purification. Process.

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Mechatronics Project EMMET System ElectroMechanical Membrane-Enabled Transfer System

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  1. Mechatronics Project EMMET System ElectroMechanicalMembrane-Enabled Transfer System Carlo Yuvienco Keeshan Williams Alberto Giacomello

  2. C. Yuvienco; K. Williams; A. GiacomelloEMMET System Objective: ProteinPurification Process

  3. C. Yuvienco; K. Williams; A. GiacomelloEMMET System PresentImplementation

  4. C. Yuvienco; K. Williams; A. GiacomelloEMMET System

  5. C. Yuvienco; K. Williams; A. GiacomelloEMMET System

  6. C. Yuvienco; K. Williams; A. GiacomelloEMMET System Vallejo, L. F. & Rinas, U. Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins. Microb Cell Fact 3, 11, doi:10.1186/1475-2859-3-11

  7. The Electrode Surface C. Yuvienco; K. Williams; A. GiacomelloEMMET System

  8. The Electrode Surface – Equivalent Circuit C. Yuvienco; K. Williams; A. GiacomelloEMMET System

  9. C. Yuvienco; K. Williams; A. GiacomelloEMMET System OurProposal

  10. C. Yuvienco; K. Williams; A. GiacomelloEMMET System Primary System Components • Digital level sensor • Reservoir • Power strip • Signal generator • Reservoir valve(servo controlled) • m controller and circuit boards • Reaction vessel • Stir bar

  11. C. Yuvienco; K. Williams; A. GiacomelloEMMET System Primary System Components • Digital level sensor • Reservoir • Power strip • Signal generator • Reservoir valve(servo controlled) • m controller and circuit boards • Reaction vessel • Stir bar

  12. C. Yuvienco; K. Williams; A. GiacomelloEMMET System Primary System Components • Digital level sensor • Reservoir • Power strip • Signal generator • Reservoir valve(servo controlled) • m controller and circuit boards • Reaction vessel • Stir bar

  13. C. Yuvienco; K. Williams; A. GiacomelloEMMET System Primary System Components • Digital level sensor • Reservoir • Power strip • Signal generator • Reservoir valve(servo controlled) • m controller and circuit boards • Reaction vessel • Stir bar

  14. C. Yuvienco; K. Williams; A. GiacomelloEMMET System I VAC Electrode Instrumentation Amplifier Current Source VDC Micro Controller A2D Converter Full Wave Rectifier Valve Actuator

  15. C. Yuvienco; K. Williams; A. GiacomelloEMMET System User Interface and Usage User is prompted to input 3-4 parameters that defines thesensitivity and operation of the system System reports measured voltage differential and status of valve and reservoir liquid level System is automated to control and maintain the concentration gradient of solutes across the membrane

  16. C. Yuvienco; K. Williams; A. GiacomelloEMMET System • Advantages • saving on time, materials, manpower • enables a precise controlof the process • completelyautomatizable, customizable • dyalisismayfollowdesiredprotocols • Future Improvements • useofIonSelectiveElectrodes • improvementof the user interface • concentrationreadings on LCD

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