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Annotated spectra for table II pS: phosphoserine pT: phosphothreonine

Annotated spectra for table II pS: phosphoserine pT: phosphothreonine p(SS) phosphorylation site localised to the two serines in brackets p(XXX) phosphorylation site localised to the residues in brackets * denotes oxidation # denotes deamidation. AVpSDIKHEVQSQLLQNEK Triply charged

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Annotated spectra for table II pS: phosphoserine pT: phosphothreonine

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  1. Annotated spectra for table II pS: phosphoserine pT: phosphothreonine p(SS) phosphorylation site localised to the two serines in brackets p(XXX) phosphorylation site localised to the residues in brackets * denotes oxidation # denotes deamidation

  2. AVpSDIKHEVQSQLLQNEK Triply charged Protein:At2g01680 Score:62 [M+3H+-98]3+ y5 y8 y6 [b10-98]2+ [b15-98]2+ b82+ b5-98 y3 y9 b3-98 y52+ y10

  3. YQp(SHS)FDDGEINPFANPTSVPAATSK Protein:At2g20840 Score:52 y6 y142+ y11 y12 [M+2H+-98]2+ y14 b11 y152+ y14 b10 y10 y102+ b15 b16

  4. MNSGSMApSPPVPEGGAGTGVVR Protein:At2g28070 Score:97 y11 [M+2H+-98]2+ y14 b12 b8-98 y9 y13 y15 y6 b21-98 y16 y17 y5 y4

  5. GAp(TS)GPLDPPAGEISR Protein:At2g35350 Score:72 b7 y8 [M+2H+-98]2+ y9 b7-98 y3 y5 y10 y2

  6. TMPSMpSPSSVSTEK Protein:At2g36330 Score:57 y112+ y122+ [M+2H+-98]2+ y8 y12 y6 y10-98 y4 y9 y9-98 y10

  7. TSEPpTPASGTSTITQR Protein:At2g38480 Score:78 y132+ [y13-98]2+ y11 y9 y13-98 y3 y13 y8 y10

  8. SSDLYGLSLpSSR Protein:At2g39660 Score:73 y7 y5 [M+2H+-98]2+ y8 b7 b5 y3 y3-98 y5-98 y7-98 y4-98 y2 b9

  9. p(SS)DLYGLSLSSR Protein:At2g39660 Score:68 [M+2H+-98]2+ y7 y5 y8 y3 y4 b9

  10. p(TSFT)LEEDPHASPLSPK Protein:At3g17650 Score:50 b8 y11 y10 b7-98 y6 y5 y8 y12 b15-98 y7 y13 y3

  11. NYDETGpSQLQASPSVVSR Protein:At3g22180 Score:80 y6 [M+2H+-98]2+ y7 y8 y10 y8-98 b12-98 b11 y3 y13 y14 y7-98 b16 b15-98 y10-98 y4 y5

  12. AVWADMGPAMpSDHGDGK Protein:At3g26935 Score:86 [M+2H+-98]2+ y10-98 y11-98 y11 y12 y10 y12-98 b5 b7 y7 b6 b15 y5 b15-98 y6 b4

  13. NAHPPEPETLDGNM*DAGAGQpTPQLR Triply charged Protein:At3g26935 Score:90 y4 y9 y7-98 [b21-98]2+ y5 b242+ y5-98 y11 b13 y3 b16 b19 b14

  14. KDEEPTPFpSGPVPR Protein:At3g27390 Score:58 [M+2H+-98]2+ y10-98 y7 y5 y8 y10 b9-98 b4 y11-98 y2 y6-98

  15. SSpSANSVTGPSTIK Protein:At3g28345 Score:38 Assignment of S3 based solely on [y12-98] 2+ Otherwise phosphorylation site can only be isolated to either S1, S2 or S3 [M+2H+-98]2+ y6 y12-98 y9 y5 y8 y10 y11 b4-98 b13-98

  16. SFpSFDVGPN#GGK # denotes deamidated Protein:At3g28850 Score:72 [M+2H+-98]2+ y6 b5 y5 y8 y10 b3-98 y9 y4 b2 y10-98 b4-98 y3

  17. ADFASAAIApTPPISK Protein:At1g01540 Score:70 y5 [b10-98]2+ y6 y8 y7 y9 b9 y10 b13 y11 b14

  18. SGPLVAQpSPDSVIVK Protein:At1g18390 Score:69 y10 y7 y10-98 y5 y8 y11 b13 b14

  19. pSGGYVGGPNEGGGGGGGWATAVR Protein:At1g30440 Score:123 [M+2H+-98]2+ y11 Y18 b19-98 b19-982+ y202+ y13 y16 y4 y17 y14 y10 Y19

  20. p(SAS)FKEESDFFADLK Protein:At1g30690 Score:72 [M+2H+-98]2+ y122+ y10 b13-98 b9 y11 b13 y9 y8 y2

  21. TLpSDPFSNGK Protein:At1g33990 Score:56 y8 [M+2H+-98]2+ y6 y2 b2 y8-98 b4 y7

  22. TDSEVTSLAASSPARpSPR (triply charged) Protein:At1g45688 Score:60 [y17-(98+18)]2+ y132+ y142+ [b4-18] y6 y3 y2

  23. NIEDIFp(SSGS)RR (doubly phosphorylated) Protein:At1g59870 Score:51 y5 [M-98-98]2+ y112+ [y8-196]2+ b4 y6 y7

  24. LpSPLPPEPVGFDYGR Protein:At1g61250 Score:73 y11 y112+ y7 y8 b4-98 y132+ y3 b3-98 b4 y10 b12 y12

  25. p(SS)PVNSGAFVSK Protein:At1g62320 Score:45 [M+2H+-98]2+ [b4-98]2+ y102+ y8 y7 y3 y10 y9

  26. TVpSDVTIGR Protein:At1g66480 Score:69 [y8-98]2+ y4 b2 [y7-98]2+ y2 b5 y5 y3 y6

  27. FHYQSGGSGGGGGAHYPAPNPGMFpSPNTGGGGGTAAK (triply charged) Protein: At1g73590 Score:74 b13-18 [M+3H+-98]3+ b202+ b232+ y12 y18-98 [b25-98]2+ y192+ b15 y162+ b18 b16 b20 y182+

  28. GPTPRPpSNYEEDGGPAKPTAAGTAAGAGR (triply charged) Protein:At1g73590 Score:64 [M+3H+-98]3+ y12 Y192+ y17 2+ b12 y9 b7-98 b13-98 b14-98 y7 b12-98 y22 2+ b5 b16-98 b16

  29. SDAVWDEPTpSPK Protein:At1g76070 Score:54 y8 y7 y9-98 b4 y5 y6 b3 b5 y9 b9 y10

  30. SEADLDSQVVDFQpSPK Protein:At1g76660 Score:92 y6 y8 y11 y5 b10 y3 b11 b8 y5-98 b9 y12 y13

  31. Dp(TT)PVAAIGK Protein:At1g77110 Score:50 [y8-98]2+ y7 [y9]2+ b3 y2 b2 y8 b5 y3 b8-98

  32. ApSWDGSLIGR Protein:At3g09070 Score:45 [M+2H+-98]2+ b8-98 y8 y6 y5 b4-98 y7 y4 y2 b3-98 y3

  33. QpTPSVQIPR Protein:At4g24630 Score:57 b5-98 y2 y4 y7 y3 y5

  34. NAISpSPYIDPR Protein:At4g32410 Score:53 y2 y6 b3 y10 2+ b8-98 y3 y9 b5-98 Y92+ y5 b9-98 b4 y4

  35. QDTSGEFSAApSPER Protein:At5g05170 Score:75 y8 y4 y6-98 y7 y6 y5-98 y4-98 y11 y9-98 y5 y3 y10

  36. pSVGSPAPVTPSK Protein:At5g12080 Score:85 y8 [M+2H+-98]2+ b4-98 y4 y6 y10 y3 y9 b3-98

  37. GGLpSPVIVPR Protein:At5g39865 Score:54 [M+2H+-98]2+ y3 b7-98 y6 b6-98 b8-98 y2 y4 y5 b4-98 y8 y7 b9

  38. QpTPTVQIPR Protein:At5g50020 Score:52 [M+2H+-98]2+ y2 y4 [y8-98]2+ y6 y7 y3 b82+ y72+ y5

  39. TLpSPKPPSPR (triply charged) Protein:At2g43680 Score:42 y82+ [b5-98]2+ [y8-98]2+ y3 y52+ y5 b2 b62+ b52+ y72+

  40. FGSAPTTAASRpSPEMK Protein:At5g67470 Score:66 [M+2H+-98]2+ y142+ y112+ y132+ b12-98 y7 y122+ y8 b14-98 y4 y8-98 y12-98 y3 y2 b14 b11 y5-98

  41. IPNpSGNLVFCGESR Protein:At5g67200 Score:105 [M+2H+-98]2+ y6 y7 y5 b4-98 y9 y11-98 y10 y8 y3 y12-98 y2

  42. QNpSENVLIDMEK Protein:At5g61740 Score:65 y4 y5 b7-18 y7 y8 b9 b11 y3 y6

  43. AVpTPSSSLEFR Protein:At5g41620 Score:66 y8 [y9-98]2+ y2 b3 y9 y9-98 y5 y3 y6 y7

  44. SVGpSPAPVpTPSK Protein:At5g12080 Score:67 [M+2H+-98]2+ y8 y3 y6 y4 y10 y7 Y10-98 b2

  45. VTVMPIIpSPERPVK Protein:At4g03390 Score:75 y132+ y102+ y10 y92+ y10-98 y6 y8-98 y7 y8 b11 y4 y3 y11

  46. FDWDHPLHLQPM*pSPTTVK (triply charged) Protein:At3g29360 Score:42 [M+3H+-98]3+ y8-98 b102+ [b13-98]2+ y8 y5 y7 b92+ y82+ b10 b4 b8 b9 y72+

  47. TV#NGGM*SpSPSLQK Protein:At3g48760 Score:97 y112+ y5 y102+ y8 y11 y9 y10 y3 y2 y6 y7 y82+

  48. SVAGGFMpSPNMGK Protein:At3g26935 Score:99 y5 y7 y6 y10-98 y10 y9-98 y11 y9 y4 y7-98 y3 y11-98 y8 y8-98 b5 y2

  49. SLFLSSTSApSPAR Protein:At3g07540 Score:83 y9 y4 y6 y8 y7 y5 y4-98 y10 y3 y2 y11

  50. Vp(SSS)PTASPTFVSTPK Protein:At2g33490 Score:57 y7 y9 y8 y12 y5 y4 y10 y3 y11 y2 y14

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