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Pg. 367 PowerPoint Presentation

Pg. 367

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Pg. 367

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  1. Pg. 367

  2. Membrane Protein Movement Tethered to other Membrane proteins Single Protein Tethered to cytoskeleton Pg. 385

  3. Cytosol Nucleus Mitochondria Chloroplasts ER Golgi Endosome Lysosome Transmembrane Secreted

  4. Cytosol Nucleus Mitochondria Chloroplasts ER Golgi Endosome Lysosome Transmembrane Secreted

  5. The Nucleus

  6. Nuclear Structure

  7. Nuclear Lamina

  8. Nuclear Structure

  9. Nuclear Pore Complex

  10. -Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val- C N Protein Transport to the Nucleus Nuclear Localization Signal (NLS) Table 15-1 (page 504)

  11. Nuclear protein with NLS removed Nucleus Nucleus NLS removed from protein Nuclear Protein with NLS NLS NLS is Necessary for Nuclear Import Pg. 504

  12. Nuclear protein with NLS removed Cytosolic Protein Without NLS Nucleus Nucleus NLS removed from protein Cytosolic Protein With NLS NLS is Sufficient for Nuclear Import Pg. 504

  13. Nuclear Import Pathway

  14. Pg. 541

  15. Regulated Nuclear Import NLS “masked” Allostery! NLS “exposed” Pg. 542

  16. Cytosol Nucleus Mitochondria Chloroplasts ER Golgi Endosome Lysosome Transmembrane Secreted

  17. ER Import Sequence Recognition Sequence N-Met-Met-Ser-Phe-Val-Ser-Leu-Leu-Leu-Val-Gly-Ile-Ile-Phe-Trp-Ala-Thr- C Hydrolysis of this peptide bond by Signal Peptidase enzyme Table 15-1 (page 504)

  18. N

  19. Membrane “budding” N C N C N C C N C N

  20. Clathrin “triskelion”

  21. Clathrin “Cage”

  22. Dynamin GTP Hydrolysis

  23. Vesicle Fusion with Membrane N C N C N C C N C N

  24. v-SNARE/t-SNARE Interaction

  25. Cytosol Membrane fusion step is regulated Secretory vesicle Ca++ Outside of cell

  26. Botulism Toxin Clostridium botulinum Protease

  27. Botulism Toxin t-SNARE (green) Botulism Toxin (tan)

  28. Botulism Toxin Botulism Toxin Protease