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Enzymatic Hydrolysis of cellulose for production of fuel ethanol by SSF

Enzymatic Hydrolysis of cellulose for production of fuel ethanol by SSF. Presented by Alaaedine Talab. What is SSF?. The reaction. The reaction continued. β(1→ 4). Purpose of SSF. Minimize Hydrolysis byproducts Economically favorable Minimize inhibitory factors Eliminate transfer time.

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Enzymatic Hydrolysis of cellulose for production of fuel ethanol by SSF

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  1. Enzymatic Hydrolysis of cellulose for production of fuel ethanol by SSF Presented by AlaaedineTalab

  2. What is SSF?

  3. The reaction

  4. The reaction continued β(1→ 4)

  5. Purpose of SSF • Minimize Hydrolysis byproducts • Economically favorable • Minimize inhibitory factors • Eliminate transfer time

  6. SSF Kinetics r1 r2 glucose cellobiose Cellulose

  7. Experimental Procedure • Effects of specific components of SSF system • Assays done in Y/P medium (10g/L yeast extract, 20g/L peptone), PH = 5.0 at 38C • TrichodermaReesei • Initial rates determined after enzyme deactivation(20 minutes boiling water, denatur) 15s intervals 1 minute duration

  8. How do we measure enzyme activity as an uncoupled system? 3g/L

  9. We can simplify the volumetric rate constants constant =

  10. Effect of cellobiose on cellulase activity

  11. Effect of cellobiose on B-glucosidase Avg. K2B?

  12. Effect of cellobiose on B-glucosidase Maximal rate

  13. Glucose concentration and enzyme activity

  14. Ethanol concentration and Enzyme activity K2E = NEGLIGIBLE

  15. Summary

  16. Putting it all together

  17. Putting it all together continued • Almost perfect fit but not yet • Why?

  18. First order rate loss added to model • Fail

  19. (Conclusion)So what? • R^2> 0.98 • Developed a quantitative mathematical model that can predict SSF progress • This applied to enzymes from T. risea however model can be used to calculate other microbe kinetics • Can be used for process, design, optimization and scale-up (industrial purpose) to enhance ethanol productivity

  20. Critique • Take into account Cellulase inactivity • Understand mechanisms of inhibition (i.e. feedback inhibition) • Use larger concentrations of substrate (max = 120g/L)

  21. References • http://www.ncbi.nlm.nih.gov/pubmed/18609632 • http://bio-process.com/wp-content/uploads/2009/12/Cellulosic-SSF.pdf

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