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Estimation of Carbohydrate by Anthrone Method

Estimation of Carbohydrate by Anthrone Method

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Estimation of Carbohydrate by Anthrone Method

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  1. Estimation of Carbohydrate by Anthrone Method • The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.

  2. H2SO4 Blue Green Complex + Anthrone Furfural * Principle

  3. Materials 1. Anthrone reagent (0.2% in conc. H2SO4 ). 2. Glucose (10mg/100ml). 3. Colorimeter or spectrophotometer.

  4. 60 5 55 10 50 45 15 40 20 35 25 30 Tube # Blank St. unk. 10 min. Boiling Water Bath Glucose (ml) 0 0.5 0.5 D. W. (ml) 1 0.5 0.5 Anthrone (ml) 4 4 4 Mix Well COOL to Room Temperature Read the Optical Density at 620 nm

  5. * Calculations Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard CUn. = A Un. × C St. A St. Samar A. Damiati