1 / 5

Estimation of Carbohydrate by Anthrone Method

Estimation of Carbohydrate by Anthrone Method . The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides. . H 2 SO 4. Blue Green Complex. +. Anthrone. Furfural. * Principle. Materials.

iain
Télécharger la présentation

Estimation of Carbohydrate by Anthrone Method

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Estimation of Carbohydrate by Anthrone Method • The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.

  2. H2SO4 Blue Green Complex + Anthrone Furfural * Principle

  3. Materials 1. Anthrone reagent (0.2% in conc. H2SO4 ). 2. Glucose (10mg/100ml). 3. Colorimeter or spectrophotometer.

  4. 60 5 55 10 50 45 15 40 20 35 25 30 Tube # Blank St. unk. 10 min. Boiling Water Bath Glucose (ml) 0 0.5 0.5 D. W. (ml) 1 0.5 0.5 Anthrone (ml) 4 4 4 Mix Well COOL to Room Temperature Read the Optical Density at 620 nm

  5. * Calculations Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard CUn. = A Un. × C St. A St. Samar A. Damiati

More Related