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Characterization of GFP-JBP1 and Mutants in JBP1 Knockout Models

This study explores the role of GFP-tagged JBP1 in JBP1 knockout (KO) models, specifically focusing on the effects of mutations H189A and D191A on protein function. The variants are analyzed for their physiological impact in various experimental conditions, with insights derived from antibody binding (α-GFP) assays. Additionally, we provide comparative data on wild-type (WT) JBP1 to elucidate the functional characteristics of the mutants. These findings are represented in Suppl. Fig. 6, contributing to the understanding of JBP1's molecular mechanisms.

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Characterization of GFP-JBP1 and Mutants in JBP1 Knockout Models

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  1. Lt GFP-JBP1 in JBP1 KO HYG : KO PUR Lt GFP-JBP1 in JBP1 KO HYG : KO PUR Lt GFP-JBP1 in JBP1 KO HYG : KO PUR H189A + D191A H189A + D191A H189A + D191A Cf JBP1 Cf JBP1 Cf JBP1 H189A D191A H189A D191A H189A D191A Lt WT Lt WT Lt WT WT WT WT - - Wells J-DNA / Lt GFP-JBP1 + -GFP J-DNA / Lt GFP-JBP1 J-DNA / Lt JBP1 J T J + -GFP Suppl. Fig. 6

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