'Gfp' diaporamas de présentation

pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP). Instructors. Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School

pGLO ™ & GFP. pGLO ™ Transformation and Purification of Green Fluorescent Protein (GFP) Instructors. Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center

pGLO plasmid. Beta Lactamase Ampicillin resistance Green Fluorescent Protein (GFP) Aequorea victoria jellyfish gene araC regulator protein Regulates GFP transcription. ara Operon. lac Operon. araC. B. A. D. LacI. Z. Y. A. Effector (Arabinose). Effector (Lactose). araC. B. A.

Purification of Green Fluorescent Protein (GFP). Instructors. Stan Hitomi Coordinator – Math & Science Principal – Alamo School San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School

http://www.elmhurst.edu/~chm/vchembook/images/566alphahelix.gif. http://en.wikipedia.org/wiki/File:Peptide_angles.png. http://en.wikipedia.org/wiki/File:Pi_helix_neg55_neg70_sideview.png. http://commons.wikimedia.org/wiki/File:Pi_helix_topview.png.

C. A. B. Propidium Iodide. Side Scatter. PI Fluorescence. GFP. GFP Fluorescence. GFP Fluorescence. Forward Scatter. E. G. D. DA6. DA5. DA7. DA8,9. F. I5. DA4. SABV(L/R). DA1. DA3. SABD.

GFP (%).

4. 4. 4. 4. 10. 10. 10. 10. 40.3. 0.1. 0.1. 0.6. 53.5. 0.1. 3. 3. 3. 3. 10. 10. 10. 10. 2. 2. 2. 2. 10. 10. 10. 10. 1. 1. 1. 1. 10. 10. 10. 10. 59.7. 37.2. 71.2. 28.1. 46.5. 62.6. 0. 0. 0. 0. 10. 10. 10. 10. 3. 3. 0. 1. 2. 4. 0. 1. 2. 4.

A Color Change Reporter System For I-SceI-mediated Genome Editing In Canine Hematopoietic Stem Cells. Nina M. Muñoz Kiem lab May 18 th , 2009. G GGATCC AC TAGGGATAACAGGGTAAT CGGTC GCCACC ATG GTG T G A T AG GGC GAG GAG. I-SceI. G GGATCC AC CGGTC GCCACC ATG GTG A G C A AG GGC GAG GAG.

Fluorescent Protein Technology. Michael W. Davidson National High Magnetic Field Laboratory and Department of Biological Science Florida State University. Seminar Objectives: Interactive Tutorials The FP C o l o r Palette: New FPs Optical Highlighter FPs

BioWire. Orr Ashenberg, Patrick Bradley, Connie Cheng, Kang-Xing Jin, Paula Nunes, Danny Popper, Sasha Rush. BioWire Outline. Overview Types of propagation Weiss Circuit Our Circuit and Timing Modeling BioBricks and Other Materials Experiments Debugging and Backup Plans Photolithography.

Innate immunity mediated by APOBEC3G.

A. MSCV. MSCV. GFP-RV. 5 ‘ LTR. IRES. GFP. 3 ‘ LTR. 3 ‘ LTR. mIFN- . IRES. GFP. IFN/GFP-RV. 5 ‘ LTR. B. GFP-RV IFN/GFP-RV. 35%. 34%. 55%. 56%. CD 8. 2%. 2%. 8%. 8%. GFP. C. GFP-RV IFN/GFP-RV. 99%. 99%.

A. 1. 25. 46. 507 aa. mTP. NLS. OsHXK5. GFP. OsHXK5-GFP. NLS. OsHXK5. GFP. OsHXK5 Δ mTP-GFP. mTP. OsHXK5. GFP. OsHXK5 Δ NLS-GFP. OsHXK5 Δ mTP Δ NLS-GFP. OsHXK5. GFP. OsHXK5NLS-GFP. NLS. GFP. B. 506 aa. 1. 29. 45. mTP. NLS. OsHXK6. GFP. OsHXK6-GFP. NLS. OsHXK6.

Asymmetric division. Text and image sources are included using the notes function of this file. All centrosomes are not equivalent!. DNA Microtubules IoDPP mRNA. Yeast spindle orientation. Video. GFP-tubulin. GFP-tubulin Dyn1:His3. Video.

PC 204 Biophysical Methods. Katalin F. Medzihradszky. Biophysical Methods. Complement X-ray and NMR structural information Measure how proteins behave in solution Can determine how they dynamically interact with each other Can determine how they interact with different substrates

Quorum Sensing: Week 6 Results. Two Cell vs. One Cell Systems. Switched Fluorophores. Original Constructs. Senders. lacI regulated RFP Sender (S08I07). tetR regulated RFP Sender (S23I07). Receiver. GFP Receiver (T02). Switched Fluorophores. Flipped Constructs. Senders.

GFP-based membrane protein overexpression and purification in E. coli and S. cerevisiae. Joy Kim. Center for Biomembrane Research Department of Biochemistry and Biophysics Stockholm University Stockholm, Sweden. Soluble protein vs. membrane protein structures. GFP-based.

Figure 1A. Vectors employed. Control line. Experimental line-1. Experimental line-2. GFP silencing reverted cells. Sf21 cell. GFP expressing cells. X. GFP silenced cells. Figure 1B. gfp shRNA Forward primer. gfp shRNA (49 bp). a. EcoRI. KpnI. 19 bp. 9 bp. 19 bp. gfp shRNA