1 / 2

Methylation Analysis in Asymptomatic Carriers of FSHD: Sodium Bisulfite Sequencing Findings

This study investigates methylation patterns in asymptomatic carriers (AS-carriers) of Facioscapulohumeral muscular dystrophy (FSHD) using sodium bisulfite sequencing. A total of 10 samples were analyzed, with the 5' region assessed in 5 samples. The analysis highlights the number of repeats and methylation status across three regions (BIS-5', BIS-Mid, BIS-3') within the D4Z4 region. Each dot in the results represents a cloned DNA molecule, indicating methylation status, with black dots for methylated CpG sites and white dots for unmethylated sites.

inez
Télécharger la présentation

Methylation Analysis in Asymptomatic Carriers of FSHD: Sodium Bisulfite Sequencing Findings

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. * BIS-Mid BSP primers BIS-5’ BIS-3’ I-I1-2 RU 60% 59% 67% C-I2-5 RU 71% 66% 55% G-I-5 RU 68% SD6-6 RU 73% BA6-6 RU D4Z4 hhspm3 DUX4 LSau 82% A F-I3-7 RU 68% A-II1-7 RU 67% 68% 71% B-I1-8 RU 78% O-I3-10 RU 64% 76% 65% O-II2-10 RU 77% 70% 58% Gaillard et al. figure e3

  2. Figure e-3: Sodium bisulfite sequencing in asymptomatic carriers not clinically affected with FSHD. For asymptomatic carriers (AS-carriers), 10 samples were investigated. For 5 of them, only the 5’ region was analyzed. The number of repeat is indicated for each sample. The position of the three different regions analyzed within D4Z4 is indicated above the corresponding column (from left to right, BIS-5’; BIS-Mid; BIS-3’). Each row of dots corresponds to a cloned DNA molecule. Black dots correspond to methylated CpG, white dots to unmethylatedCpGs and absence of dot represents sequence variation compared to the reference sequence. The percentage of methylated CpG among all CpGsanalyzed/individual is given below each sample.

More Related