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Analysis of Chimaeric PVX Vectors in N. benthamiana Leaves Reveals Marker Expression Patterns

This study examines the infiltration of chimaeric PVX vectors in N. benthamiana leaves at different time points to analyze marker expression patterns. The systemic leaves were also evaluated for marker presence using PVX-S6, PVX-S7-1, PVX-S7-2, PVX-S8, PVX-S9-1, PVX-S9-2, and PVX-S10 constructs. Immunoblotting with CP antiserium detected 34 kDa and 26 kDa bands. Detailed results are presented in Figure S2A and B.

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Analysis of Chimaeric PVX Vectors in N. benthamiana Leaves Reveals Marker Expression Patterns

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  1. N. Benthamiana leaves infiltrated with chimaeric PVX vectors ( 7 dpi) PVX PVX-S6 PVX-S7-1 PVX-S7-2 PVX-S8 PVX-S9-1 PVX-S9-2 PVX-S10 Agro-infiltrated leaves Systemic leaves S7-1 PVX Marker Marker S7-2 Mock Mock S9-1 PVX S6 S9-2 S6 S9-2 S7-2 S10 S10 S7-1 S8 S8 S9-1 34 kDa 26 kDa PVX CP antiserium loading N. Benthamiana leaves infiltrated with chimaeric PVX vectors (15 dpi) PVX PVX-S6 PVX-S7-1 PVX-S7-2 PVX-S8 PVX-S9-1 PVX-S9-2 PVX-S10 Systemic leaves PVX Marker S7-2 S9-1 S7-1 S6 S9-2 S10 S8 34 kDa PVX CP antiserium loading Fig. S2 A B

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