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Proteins: Structure, Chemical Identification & Polypeptide Synthesis

This guide presents an overview of proteins, their structure, and methods for chemical identification through the Biuret test. Learn about the role of amino acids as monomers forming polypeptide chains and observe the reaction between Biuret solution and amino acids in proteins, where a color change from blue to violet indicates protein presence. Additionally, understand the process of dehydration synthesis in creating polypeptides and follow essential lab safety practices for conducting these experiments. Conclude with observations and implications of your findings.

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Proteins: Structure, Chemical Identification & Polypeptide Synthesis

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  1. Proteins: Structure, Chemical Identification & Polypeptide Synthesis Using Biuret to Identify Presence of a Protein Learning the Chemical Structure of an Amino Acid Using a Dehydration Synthesis to Make a Polypeptide Chaine

  2. Testing for Organic CompoundsPart A: Protein Testing 1. Involves an indicator, Biruet Solution 2. Biuret reacts with the amino acids in the protein 3. Solution color goes from blue to violet 4. Color change indicates a chemical reaction between the biuret and the bonds between the amino acids of a protein

  3. Another Look at Biuret Test Postive Control Experiment Negative Control Chemistry of the Biuret Test

  4. Proteins • Are organic polymers composed of monomers called amino acids. • Strands of amino acids are called polypeptide chains. Where are the peptide bonds? Proteins are many chained structures.

  5. Polypeptide Chain Amino Acids Protein with two Polypeptide chains Where is the complete protein?

  6. Parts of an Amino Acid

  7. Here is another diagram of an amino acid….

  8. Lab Safety & Cleanup • Test tubes are glass. • Biruet Solution is a base. Wash hands with water. • Goggles on at all times • Use special glassware soap and water with test tube brush to wash all test tube • Return all materials to appropriate location • Complete data table. • On back of lab, construct (5) observation statements and then write a conclusion.

  9. Polypeptide Synthesis • Involves two amino acids. • Involves a dehydration synthesis. • Involves a chemical reaction that occurs between two specific areas of the amino acid. • Requires an –OH group and an –H from another –OH group

  10. Functions of Proteins http://www.youtube.com/watch?v=iB7B0YaSLhU

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