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WP7: Novel products and processes

WP7: Novel products and processes. Nico Boon Baharak Hosseinkhani Laboratory of Microbial Ecology and Technology (LabMET) Ghent University Belgium. Work package 7: establishing new product/processes for bioremediation. ETS. IAMC. UGENT. UNIBO.

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WP7: Novel products and processes

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  1. WP7: Novel products and processes Nico Boon Baharak Hosseinkhani Laboratory of Microbial Ecology and Technology (LabMET) Ghent University Belgium

  2. Work package 7: establishing new product/processes for bioremediation ETS IAMC UGENT UNIBO

  3. Task 7.1. Using hydrocarbon-degrading enzymes and microorganisms to obtain valuable chemicals (fine chemicals, biosurfactants) - UNIBO • Selection of the most promising biosurfactant-producing isolates obtained in WP4 (by TUC) through comparison of their ability to grow and accumulate crude biosurfactants in the presence of weathered crude oilin 1L stirred tank bioreactor under batch conditions • Analysis of the potential environmental impact of the selected most promising biosurfactants by assessment of their ecotoxicity through an ecotoxicological biotest based on the use of the bioluminescent bacterium Vibrio fischeri Start of the activities: June 2012

  4. Task 7.2. Development of small scale bioprocesses for the reductive dehalogenation of chlorinated compounds - UNIBO • Testing the most promising selected microbial consortia for the reductive dechlorination of PCBs occurring in actual site sediments of Porto Marghera (Venice Lagoon) area in slurry-phase 2 L anaerobic bioreactors at ambient temperature • Investigation of the effect of different factors (inoculum size, different types/amounts of electron donors, biosurfactants) on the biotransformation of PCBs in the reactors. Start of the activities: February 2013

  5. Task 7.2. Development of small scale bioprocesses for the reductive dehalogenation of chlorinated compounds - UMIL • Reactivation of microcosms where a new dehalogenase enzyme potentially involved in 1,2-dichloroethane (1,2-DCA) reductive dechlorination was previously detected • Preparation of DCA containing microcosms from polluted sediments collected by different ULIXES partners and sent to UMIL during the second semester of the project. These sediments were collected from: • Augusta bay • Haven shipwreck • Messina polluted sand • El-Max district (Alexandria, Egypt) • Aqaba Gulf • Progressive adaptation of the culture to salinity is ongoing • Set-up of an analytical method based on gas chromatography • to evaluate the DCA removal by the microbial consortia

  6. Task 7.3: Novel biopalladium nanoparticles technology for dechlorination processes - UGent Concomitant biopalladium and H2 producing strains obtained from WP4 will used for: • Reductive dehalogenation of PCB and Cl-solvents in microcosms (focus on brackish/salt) • Bioreactor and Venice lagoon sediment • Optimization of Bio-Pd sizes and reactivity of catalysts for dechlorination process • Extra :develop a recovery option of Bio-Pd after application in situ • Extra: preparing Novel generation of palladium based bimetallic nanoparticle (Pd/Au , Pd/Fe)and investigation of their ability for removing and dechlorination of PCB/Cl-solvent Start of the activities: June 2012

  7. Task 7.4: Fate of metal/metalloids in sediments - ETS Selected sediment samples (WP2) were used for the preparation of microcosm dedicated for time-course experiments. In microcosms, the biodegradation yields of organic contaminants by the autochthonous microbial consortia will be tested and optimized through anaerobic incubations and different environmental conditions. Different endpoints were selected for each experimental set, in order to monitor the changing of micrbial growth and diversity together with hydrocarbon degradation rates and heavy metal partitioning Start of the activities: 2012

  8. Task 7.4: Fate of metal/metalloids in sediments - ETS Experimental microcosms Factorial experimental design: • Anaerobic incubations of 250 mL slurries prepared with 25% of homogenized sediment and 0.2 μm prefiltered seawater, on continuous rotary shacking. • Controlled temperatures (25°C and 15°C) • Incubation times: 0, 28, 60 days • Presence/absence of nutrients (C:N:P=100:10:1 molar) • Presence/absence of substrate (Lactose/acetate, 20 mM of C)

  9. Task 7.4: Fate of metal/metalloids in sediments - ETS Experimental microcosms In each microcosms, the following parameters will be monitored at each incubation time: • pH • Eh • Total prokaryotic abundance, biomass and diversity • Heavy metal concentration (overlying water) • Heavy metal concentrations and partitioning in the diffrent geoghemical phases • Hydrocarbon concentrations

  10. Time table WP7 Start month: 12; End month: 35

  11. Points of discussion for WP7?

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