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How to Separate These Objects

How to Separate These Objects . 1. 3. 2. 6. 8. 5. 4. 9. 7. 10. 11. 12. 1. 3. 2. cotton. 8. 4. wood. 5. 6. 8. 4. 7. 5. stone. 9. 10. 11. 12. Shape Size Density. wood stone cotton wood wood cotton stone wood stone cotton stone cotton. Shape. 4. 6. Size.

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How to Separate These Objects

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  1. How to Separate These Objects 1 3 2 6 8 5 4 9 7 10 11 12 1 3 2 cotton 8 4 wood 5 6 8 4 7 5 stone 9 10 11 12 Shape Size Density wood stone cotton wood wood cotton stone wood stone cotton stone cotton Shape 4 6 Size 8 5 Density 7 Different rolling speed Different sedimentation Sieving different sizes Juang RH (2004) BCbasics

  2. Basic Principles of Protein Purification Cell Macromolecule Organelle Homogenization Small molecule Cell Debris Amino acid, Sugar, Nucleotides, etc Protein Nucleic acid Carbohydrate (Lipid) Ammonium sulfate fractionation Size Charge Polarity Affinity Ion exchange, Chromatofocusing, Disc-PAGE, Isoelectric focusing Reverse phase chromatography, HIC, Salting-out Gel filtration, SDS-PAGE, Ultrafiltration Affinity chromatography, Hydroxyapatite Juang RH (2004) BCbasics

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