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Impact of Roc-A and Roc-Q on Apoptotic and Survival Cells in MFL-3 Culture

Investigate the effects of Roc-A and Roc-Q treatment on apoptotic and survival cells in MFL-3 culture. Quantify apoptotic cells at 16 hours post-treatment and assess survival cells based on FSC/SSC profiles. Compare responses to PMA/iono, ConA, and different concentrations of Roc-A and Roc-Q. Visualize results with FLIPL and Tubulin markers. Data based on Zhu et al.'s study (Fig. S2).

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Impact of Roc-A and Roc-Q on Apoptotic and Survival Cells in MFL-3 Culture

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  1. RMA RMA 60 120 50 100 40 80 % Apoptotic cells (16 h) % Survival cells (FSC/SSC) 30 60 20 40 10 20 0 Roc-Q (nM): 0 0 50 50 0 0 0 + + + Roc-A (50 nM): Roc-A (nM): 0 0 0 0 50 50 + + + + PMA/iono.: - + - + - + ConA (5 mg/ml): + + MFL-3 (5 mg/ml): C PMA/ionomycin Con A Roc-Q Roc-Q Roc-A Roc-A no no - FLIPL - Tubulin Fig. S2. (Zhu et al.) A B

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