'Apoptotic cells' diaporamas de présentation

Apoptotic Cell Clearance and the Resolution of Inflammation

Apoptotic Cell Clearance and the Resolution of Inflammation. Jeremy Hughes MD PhD Wellcome Trust Senior Research Fellow in Clinical Science MRC Centre for Inflammation Research, University of Edinburgh. Talk Outline: Apoptosis in inflammation Current apoptotic cell recognition mechanisms

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Figure 17.1 Apoptosis

Figure 17.1 Apoptosis. Figure 17.2 Phagocytosis of apoptotic cells. Key Experiment 17.1: Photomicrographs of a normal worm (A) and a ced-3 mutant (B). Figure 17.3 Programmed cell death in C. elegans. Figure 17.4 Caspase targets. Figure 17.5 Caspase activation.

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Chapter 18 Apoptosis

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FACS Flourescens Activeted Cell s ortering

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FACS Flourescens Activeted Cell s ortering

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Cell viability studies

Cell viability studies. Sepideh Khoshnevis. The Goal. To distinguish live cells from dead and apoptotic cells in order to calculate the the percentage of viable cells for each experiment. Cell viability study. Trypan blue studies Finding data for a small population

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340 views • 22 slides

Complement system & activation Dr.Ghada Boghdadi

Complement system & activation Dr.Ghada Boghdadi. Objectives: List the components of the complement system. Discuss the chemical composition of complement. Explain the role of host defense mechanisms. Explain the role of complement in disease process.

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Legends for supplementary figures

Legends for supplementary figures

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Simultaneous Measurement of Necrotic,

Simultaneous Measurement of Necrotic,. Apoptotic Dead and Live Cells. Necrotic/Apoptotic Dead. Treat Cells with DNAse to nick cells. Incorporate FITC-UTP with TdT. Apoptotic Live. Fix cells and permeabilize. Incorporate B-UTP with TdT then. PE-Avidin. SIMULTANEOUS EVALUATION OF

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star

Volle_FigS1. SHP L2/L2. SHP L2/L2. SHP L2/L2. SHP L2/L2. SHP L2/L2. SHP L-/L-. SHP L-/L-. SHP L-/L-. SHP L-/L-. SHP L-/L-. a. b. c. 6. 6. 80. 5. *. 4. 60. 4. Relative mRNA expression. Fold induction. Fold induction. 3. 40. 2. *. 2. 20. 1. 0. 0. 0. 28. 30. 28.

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PARP

A. B. 0 0.5 1 2.5 5 mM. PARP. % of apoptotic cells. 1.0 0.77 0.7 0.33 0.30 fold. Actin. Conc. (mM). C. D. 0 2.5 5 mM. 0 0.5 1 2.5 5 mM. Bcl-2. AIF. 1 0.79 1.06 fold. 1 1.01 0.68 0.3 0.43 fold.

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100

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S3 IETD

Supplemental Data Figure 2. 20. 15. Apoptotic cells (%). 10. 5. WT. S3. S3 IETD. S3 ZB4. S3 CH11. S3 CH1 IETD. S3 CH11 ZB4. S3 DMSO.

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% Apoptotic cells (16 h)

RMA. RMA. 60. 120. 50. 100. 40. 80. % Apoptotic cells (16 h). % Survival cells (FSC/SSC). 30. 60. 20. 40. 10. 20. 0. Roc-Q (nM):. 0 0 50 50 0 0. 0. +. +. +. Roc-A (50 nM):. Roc-A (nM):. 0 0 0 0 50 50. +. +. +. +. PMA/iono.:. - + - + - +.

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Control

a. *. *. a. a. 0 hours 72 hours. Control. Apoptotic Cells. Apoptotic Cells +Taxol+CoQ+Zn 2+. b. Quantification of attached HeLa apoptotic cells. 1200. 1000. Cell number. 800. 0 hours. 600. 24 hours. *. 400. *. 48 hours. *. 72 hours. 200. 0. c.

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control

necrotic cells. necrotic cells. 100%. non-apoptotic cells. non-apoptotic cells. early apoptotic cells. early apoptotic cells. late apoptotic cells. late apoptotic cells. 50. 24 hours. 45. 80%. 48 hours. 40. 35. 30. 60%. Percent of G44 cells. G44 proliferation (cell number*10^4).

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