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This supplemental figure illustrates the analysis of HBZ-C/EBPa cell lysates tagged with MycHis and FLAG. The experiment demonstrates the successful expression and purification of tagged proteins, allowing for subsequent functional assays and interaction studies. Detailed methodology includes lysis conditions, purification procedures, and validation tests, emphasizing the significance of tagging in enhancing protein detection and characterization. This work aids in understanding the roles of HBZ-C and EBPa in cellular processes and potential therapeutic applications.
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